摘要
利用我国自有资源巴马小型猪群,完成了疫病净化,建立了无特定病原体巴马小型猪实验猪群。制定了饲育标准、实验猪遗传学检测标准和微生物质量控制技术标准,制订了黑龙江省地方标准《无特定病原体猪微生物学监测技术规范》,对建立的猪群进行了遗传学和微生物质量控制。建立了猪瘟病毒(CSFV)和高致病性猪繁殖与呼吸综合征病毒(PRRSV)感染巴马小型猪感染模型。扩增了巴马小型猪重要细胞因子及固有免疫相关的重要分子,从分子水平进一步证明巴马小型猪可以代替家猪进行猪病感染实验。目前建立的猪群已广泛应用在猪瘟病毒、猪繁殖与呼吸综合征病毒、猪传染性胃肠炎病毒、猪圆环病毒等猪重要疫病防控及致病机理的研究,实现了共享利用。本项目的完成解决了实验用猪瓶颈问题,提升了动物疫病研究水平,提供了新型实验动物,实现了优质种质资源共享利用,加速了生命科学研究和生物制品产业发展,推动了农业实验动物的行业进步。
In this study, a Specific Pathogen-Free （SPF） Bama miniature pig herd was established.The standards for breeding experimental pigs, genetic test and microbiological quality control had been drafted.The local standards of Heilongjiang province ＂SPF＂ technical specifications for microbiological monitoring of pigs had been formulated.The genetic and microbiological quality criterion had been used to control of the SPF pig herd.Classical swine fever virus （CSFV） and highly pathogenic porcine reproductive and respiratory syndrome virus （PRRSV） infection models of Bama miniature pigs were established, especially the infection miniature pig model of PRRSV was used to evaluated commercially vaccine.The comparison of the cytokine homology between Bama miniature pigs and domestic pigs showed that the Bama miniature pigs can be used to instead of domestic pigs as an ideal experiental animal.At present the SPF Bama miniature pig colonies have been widely used to study the mechanism of prevention and pathogenic in the classical swine fever virus, porcine reproductive and respiratory syndrome virus and porcine transmissible gastroenteritis virus and porcine circovirus and so on.The completion of the project solved the bottleneck problem of the experimental pig usage which provided a new ideal experimental animal for animal disease and life science research.
出处
《中国比较医学杂志》
北大核心
2017年第5期1-3,共3页
Chinese Journal of Comparative Medicine
基金
中央级公益性科研院所基本科研业务费专项(2008-04
2009-15)
黑龙江省地方标准项目(2014-38)
黑龙江省科技攻关(PC08S01)
关键词
巴马小型猪
无特定病原体
培育
感染模型
Bama miniature pig
Specific Pathogen-Free
Breeding
Infection model
