摘要
为探究乳酸菌等益生菌在雏鸡抗柔嫩艾美耳球虫(Eimeria tenella)感染中的作用,本研究以150只1日龄肉雏鸡为试验动物,以2×10~8CFU/200 L鼠李糖乳杆菌、乳酸片球菌和植物乳杆菌复合制剂饲喂雏鸡,并设红、白对照组。于攻虫前后检测细胞因子IFN-γ和IL-2、IgG和SIgA水平,雏鸡血液中淋巴细胞增殖情况和外周血CD4^+和CD8^+T淋巴细胞数量,并对攻虫期间增重情况、卵囊排出数量和盲肠病变情况进行检测。结果显示,该制剂显著提高了IFN-γ、IL-2、IgG、SIgA水平和外周血中T淋巴细胞数量,从而增强了机体细胞和体液免疫水平(P<0.05)。此外,该制剂可以显著减轻球虫感染对雏鸡增重的影响,降低卵囊排出量和盲肠病理损伤(P<0.05),ACI值达到中等抗球虫效果,有望成为鸡球虫病新的防治手段。
Abstract： In order to investigate the role and immune effects of Lactobacillus against Eimeria tenella in broiler chickens,150 one day-old broiler chickens were randomly divided into three groups in this study. One group was feed with 2×10^8CFU/200 L compound preparation（Lactobacillusrhamnosus, Pediococcus acidilactici,Lactobacillus plantarum）,the negative and positive control groups were set at the same time. The levels of cytokines IFN-γ,IL-2,IgG and SIgA,the proliferation of lymphocytes and the number of CD4^＋ and CD8^＋ T lymphocytes in peripheral blood were detected before and after challenge with E. tenella oocysts. Additionally,the body weight gain,the number of oocysts shedding and cecal le- sion also were detected. The results indicated that the preparation enhanced the cellular and humoral immunity by significantly increasing IFN-γ,IL-2,IgG,SIgA levels and the number of T lymphocytes in pe- ripheral blood（P〈0.05）.Furthermore,theeffectofweightlossonchickensinfected withEimeria oocysts, the number of oocysts shedding and cecal pathological injury were reduced significantly by the com- pound preparation（P〈0.05）. Therefore,it concludes that probiotics is expected to become a new preven- tive method of chicken coccidiosis.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2017年第6期733-738,共6页
Chinese Veterinary Science
基金
国家高技术研究发展计划(863)项目(2013AA102806)
国家自然科学基金项目(31272552
31272541
31672528)
吉林省科技发展计划项目(20160519011JH)
吉林省产业创新专项资金项目(2016C063)
关键词
柔嫩艾美耳球虫
乳酸菌
细胞免疫
体液免疫
保护效果
Eimeria tenella
Lactobacillus
cellular immunity
humoral immunity
protective effect
