摘要
目的探讨低强度激光修复对巴马猪退变椎间盘炎症因子表达的影响。方法 9头巴马小型猪,随机分为对照组(Con组)、造模组(Mod组)及激光治疗组(Las组),每组各3头。Con组不进行任何处理,Mod组及Las组在影像引导(DSA)定位引导下利用16G针刺入猪L2-3、L3-4、L4-5、L5-6椎间盘进行破坏,建模后1个月,Las组对已处理的椎间盘进行低能量激光治疗,其他两组不进行治疗.3组观察1个月后放血处理,取出椎间盘,行HE染色检测其形态学变化,免疫组化染色检测间盘肿瘤坏死因子α(TNF-α)、白细胞介素-1(IL-1)表达的变化。结果 Con组猪的椎间盘T_加权像呈高信号,Mod组椎间盘髓核T_加权信号强度明显下降,椎间盘信号为灰黑色或者黑色的低信号影,且椎间隙变窄;Las治疗组T_加权像呈中、高信号影。与Con组比较,Mod组和Las组的TNF-α、IL-1的表达均显著升高,差异有统计学意义(P<0.05);与Mod组比较,Las组TNF-α、IL-1含量均显著降低,差异有统计学意义(P<0.05)。结论低能量激光修复术对退变的猪椎间盘具有一定的修复作用,其作用机制可能与抑制炎症反应有关。
Objective By building a miniature swine model of intervertebral disc degenerative diseases, to observe low intensity laser repair of bama pig inflammation tissue factor expression in degeneration. Methods A total of 9 miniature pig were randomly divided into the control group （Con group）, laser module group （Mod group）and laser treatment group （Las group）, with 3 in each group. Pigs in the Con group were without any treatment,in Mod group and Las group,under DSA positioning guide, 16 g needle was put into pigs on intervertebral disc of L2-3 ,L3-4,L4-5 and L5-6 to make damage,1 month after modeling,the intervertebral disc in Las group were treated by low energy laser, the other two groups were not treated. Three groups were bleeding after 1 month treatment, the disc was taken out, then test their morphological changes by HE staining, and immunohistochemical detection of dish of TNF-α and IL-1 expres- sion changes. Results MR testing results showed that in the Con group of intervertebral disc T2 weighted images showed high signal, in the Mod group of intervertebral disc nucleus pulposus T2 weighted signal intensity reduced obviously,intervertebral disc signal for a film or low signal of black shadow, and intervertebral disc became narrow ; in Las group, the T＇2 weighted images showed middle and high signal. Compared with Con group, the expression level of TNF-α and IL-1 in Mod group and Las group significantly increased, and the difference was statistically significant（P 〈 0. 05 ） ;compared with the Mod group, the expression level of TNF-α and IL-1 in I.as group significantly reduced, and the difference has statistical significance （P 〈 0. 05 ）. Conclusion Low energy laser repair of inter- vertebral disc degeneration of pigs has certain repair effect, its mechanism may be related to inhibiting inflammatory reaction.
出处
《临床军医杂志》
CAS
2017年第5期493-496,500,共5页
Clinical Journal of Medical Officers
基金
沈阳市科技计划项目(F14-158-9-11)
关键词
低强度激光
椎间盘退化
炎症因子
Low intensity laser
Intervertebral disc degeneration
Inflammatory cytokines
