摘要
从进境的美国高粱样品中分离到一株与葡萄茎枯病菌Didymella glomerata相似的菌株4358-17。其菌落形态和显微特征均与葡萄茎枯病菌一致。多个位点(LSU、ITS、TUB2、ACT)序列比对显示菌株4358-17与GenBank登录号为KT389718、FJ427004、FJ427115、FJ426896的葡萄茎枯病菌相似性均达到100%。系统发育分析结果表明菌株4358-17与葡萄茎枯病菌株聚集在同一个分支上,支持率为99%。菌株4358-17接种高粱和小麦叶片,4d后接种部位出现明显症状。根据上述试验结果,将进境美国高粱样品中分离的菌株4358-17鉴定为葡萄茎枯病菌。
The isolate 4358-17 was obtained from imported Sorghum bicolor from America.It was similar to Didymella glomeratain morphology.BLAST analysis indicated that LSU,ITS,TUB2 and ACT sequence showed 100% identity with the sequences with accession numbers of KT389718,FJ427004,FJ427115 and FJ426896,respectively.Phylogenetic analysis revealed that the isolate 4358-17 clustered within D.glomeratastrains.Pathogenicity test showed that it could cause leaf spot on sorghum and wheat 4 d after inoculation.Based on all above results,the isolate 4358-17 was identified as D.glomerata.
出处
《植物保护》
CAS
CSCD
北大核心
2017年第3期192-198,共7页
Plant Protection
基金
国家质量监督检验检疫总局科技计划(2012IK278)
"十二五"国家科技支撑计划(2012BAK11B03)
关键词
葡萄茎枯病菌
形态特征
系统发育分析
致病性测试
Didymella glomerata
morphological characters
phylogenetic analysis
pathogenicity test
