摘要
目的:建立胃痛丸中柚皮苷、新橙皮苷的高效液相色谱法(HPLC)含量测定方法。方法:采用HPLC,色谱柱为Agilent TC-C18柱,以乙腈-水(20∶80)为流动相,流速为1.0ml·min-1,检测波长为283 nm,柱温为30℃。结果:柚皮苷和新橙皮苷分别在0.027~4.552μg(r=0.999 9)、0.029~4.016μg(r=0.999 8),进样量与峰面积呈良好的线性关系,平均加样回收率分别为102.19%、103.60%,RSD分别为2.88%、1.79%(n=9)。结论:方法简便、准确、灵敏、重复性好,可作为胃痛丸中的柚皮苷和新橙皮苷含量控制方法。
Objective ： To establish a method for the content determination of naringin and ueohesperidin in Weitong pills. Meth- ods： An HPLC method was adopted. The determination was performed on an Agilent TC-Cts column with the mobile phase consisting of acetonitrile-water（20：80） at the flow rate of 1.0 ml ·min-1. The detection wavelength was set at 283nm. The column temperature was 30 ℃. Results： Naringin and neohesperidin respectively within the concentration range of 0. 027-4. 552 μg （r = 0. 999 9 ） and 0. 029-4. 016 μg （r = 0. 999 8 ） had good linear relationship with the peak area, the average recovery was 102.19% and 103.60% , and the RSD was 2.88% and 1.79% （ n = 9）, respectively. Conclusion： The method is simple, accurate and reproducible, and suitable for the content determination of naringin and neohesperidin in Weitong pills.
作者
彭玲娜
丁野
孙辉
李文莉
罗疆南
Peng Lingna Ding Ye Sun Hui Li Wenli Luo Jiangnan(Hunan Institute for Drug Control, Changsha 410001, China)
出处
《中国药师》
CAS
2017年第6期1112-1114,共3页
China Pharmacist
基金
国家药典委员会国家药品标准提高行动计划项目(编号:59)
