摘要
以N-异丙基丙烯酰胺(NIPAM)和丙烯酸丁酯(BA)的共聚物为壁材,采用乳液聚合法制备了温度响应型吡唑醚菌酯微囊。通过光学显微镜、扫描电镜、激光粒度分析仪和紫外分光光度计等对该微囊的形貌、粒径、包封率和载药量进行表征,同时采用透析袋法探讨其释放性能,并以斑马鱼为试材测定其对水生生物的急性毒性。结果表明:吡唑醚菌酯微囊呈球形,平均粒径为1.04μm,包封率为78.30%,载药量为15.66%。吡唑醚菌酯微囊具有明显的温度响应性特征,其低临界溶解温度(LCST)为28.2℃,当环境温度高于28.2℃时能够快速释放活性成分,而低于该温度时其释放行为受到抑制。吡唑醚菌酯微囊对斑马鱼急性毒性的LC50(96 h)值为有效成分4.48 mg/L,较吡唑醚菌酯原药的提高了90倍以上,因此能够显著提高吡唑醚菌酯对水生生物的安全性。
Thermo-sensitive pyraclostrobin microcapsules were obtained via emulsion polymerization successfully using poly（N-isopropylacrylamide-co-butylacr-ylate） as wall material. The morphology, the average diameter, the drug loading and the encapsulation efficiency of microcapsules were characterized by scanning electron microscope, optical microscope, automatic laser granularity analyzer, ultraviolet spectrophotometer. The performance of its controlled-release was studied by dialysis bag method. The acute toxicity of thermo-sensitive pyraclostrobin microcapsules to Brachydanio rerio was determined. The results showed that the obtained microcapsules had a smooth and spherical surface, and average diameter of thermo-sensitive pyraclostrobin microcapsule was 1.04 μm. The drug loading and encapsulation efficiency of thermo-sensitive pyraclostrobin microcapsules were 15.66% and 78.30% , respectively. The results also showed that pyraclostrobin microcapsules had representative temperature sensitivity, and the responsive temperature of microcapsule was 28.2℃. When the environment temperature was higher than 28.2℃, the microcapsule could release the active ingredient rapidly. When the environment temperature was lower than 28.2℃, its release behavior was restrained. The LC50 （96 h） value of thermo-sensitive pyraclostrobin microcapsules was 4.48 mg/L （active ingredient）, it rised the LC50 （96 h） value of pyraclostrobin by 90 times, which significantly improved the security of pyraclostrobin to aquatic organisms.
出处
《农药学学报》
CAS
CSCD
北大核心
2017年第3期381-387,共7页
Chinese Journal of Pesticide Science
基金
化学肥料和农药减施增效综合技术研发专项(2016YFD0201200)
中国烟草总公司科技重点项目(110201302014)
湖南省高校重点实验室平台开放基金(15K064)
湖南省研究生科研创新项目(CX2016B304)
关键词
温度响应型微囊
吡唑醚菌酯
N-异丙基丙烯酰胺
丙烯酸丁酯
乳液聚合法
透析袋法
thermo-sensitive microcapsules
pyraclostrobin
N-isopropylacrylamide （NIPAM）
butyl acrylate （BA）
emulsion polymerization
dialysis bag method
