摘要
目的观察分析雷公藤多苷改善糖尿病肾病大鼠肾性炎性损伤的保护效果。方法选择75只雄性SD大鼠作为研究对象,包含10只大鼠为对照组,另外65只大鼠为模型组,通过腹腔注射链脲佐菌素建立糖尿病肾病大鼠模型,共64只大鼠成功,并采用随机数字抽取表法将成功的大鼠分为模型组、雷公藤多苷组和罗格列酮组,雷公藤多苷组又分为9mg/kg、18mg/kg。观察分析给药后不同组大鼠的尿素氮、血肌酐、24h尿蛋白量、血清中IL-1β水平,血清IL-17水平、IFN-y水平。结果模型组与对照组相比,其尿素氮、血肌酐、24h尿蛋白量含量、血清IL-1β、血清IL-17、IFN-y水平含量显著升高,数据比较差异具有统计学意义(P<0.05);9mg/kg雷公藤多苷组、18mg/kg雷公藤多苷组和罗格列酮组与模型组相比,尿素氮、血肌酐、24h尿蛋白量含量显著降低,数据比较差异具有统计学意义(P<0.05)。18mg/kg雷公藤多苷组和罗格列酮组与模型组相比,血清IL-1β、血清IL-17、IFN-y水平显著降低,数据比较差异具有统计学意义(P<0.05)。结论雷公藤多苷改善糖尿病肾病大鼠肾性炎性损伤效果显著,能够明显降低血清和肾脏炎性因子水平,实现对肾脏的保护,具有较高的研究价值。
Objective To observe the protective effect of tripterygium glycosides on renal inflammatory injury in diabetic nephropathy rats. Methods 75 male SD rats were used as research materials, including 10 rats in control group and 65 rats in the model group by intraperitoneal injection of streptozotocin diabetic nephropathy rats, and 64 rats were successful. According to the random number table method, the success of extraction the rats were randomly divided into model group, tripterygium glycosides group and rosiglitazone group, and tripterygium glycosides group was divided into 9mg/kg and lgmg/kg. The different groups of rats after treatment on urea nitrogen, serum creatinine, 24h urine protein, serum IL-1β levels, serum IL-17 levels, IFN-y levels were observed and analyzed. Results Comparing with the control group, the blood urea nitrogen, creatinine and 24h urinary protein content, serum IL-1β, serum IL-17 and beta IFN-y levels of the model group increased significantly, and there was statistically significant difference （ P 〈 0.05）. The 9mg/kg tripterygium glycosides group, 18mg/kg group tripterygium glycosides group and rosiglitazone group was compared with the model group, the urea nitrogen, serum creatinine and 24h urine protein content decreased significantly, and there was statistically significant difference （ P 〈 0.05 ）. Comparing with the model group, serum IL-1β, serum IL-17 and IFN-y levels of the tripterygium glycosides group and rosiglitazone group were significantly lower than those of the model group （ P 〈 0. 05 ）. The difference was statistically significant （ P 〈 0.05 ）. Conclusion The tripterygium glycosides can improve renal inflammatory injury in diabetic nephropathy rats, can significantly reduce the level of inflammatory cytokines in serum and kidney, and has a high research value.
出处
《光明中医》
2017年第11期1577-1579,共3页
GUANGMING JOURNAL OF CHINESE MEDICINE
关键词
雷公藤多苷
糖尿病肾病
炎性损伤
实验研究
Tripterygium glycosides
Diabetic nephropathy
Inflammatory injury
Experimental study
