摘要
研究从患溃疡症斑点叉尾鲙中分离到一株致病性菌株ry01,生理生化鉴定和16S rDNA基因序列分析表明ry01株为鲑气单胞菌无色亚种(Aeromonas salmonicida subsp.achromogenes)。人工感染该菌后发病鱼表现为与自然发病类似症状,且从组织中再分离的细菌特性与原感染菌相同。腹腔注射后该菌株对斑点叉尾鲙的半数致死量为4.17×10~6 CFU/m L。菌株ry01对强力霉素及左氧氟沙星等高度敏感,为斑点叉尾鲙该病防控提供了理论依据。
The isolated bacterial strain ry01 from Ictalunes punctatus with ulcer disease was identified as Aeromonassalmonicida subsp. achromogenes by analyzing biochemical reactions and 16S rDNA gene sequence. The ry01-injec-ted fish showed similar morphological and biochemical characteristics to those from naturally infected fish, supportingthat Aeromonas salmonicida subsp. achromogenes was the pathogen to Ictalunes punctatus. The LD50was 4.17×106 CFU/mL according to the Reed-Muench method. The isolated bacterial strain ry01 was highly sensitive to doxycycline andlevofloxacin. This study provides theoretical basis for the prevention and control the disease of Ictalunes punctatus.
作者
杨移斌
胥宁
董靖
杨秋红
刘永涛
艾晓辉
YANG Yi-Bin XU Ning DONG Jing YANG Qiu-Hong LIU Yong-Tao AI Xiao-Hui(Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China Hubei FreshwaterAquaculture Collaborative Innovation Center, Wuhan 430223, China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2017年第4期787-792,共6页
Acta Hydrobiologica Sinica
基金
公益性行业(农业)科研专项(201203085)资助~~
关键词
斑点叉尾鲙
杀鲑气单胞菌无色亚种
分离鉴定
药敏特性
Ictalunes punctatus
Aeromonas salmonicida subsp. achromogenes
Isolation and identification
Antimicrobial susceptibility
