摘要
探讨牛磺酸(taurine,Tau)通过p-p38通路对牛肺主动脉内皮细胞(PAECs)中ICAM-1,VCAM-1的影响及其作用机制。原代培养PAECs取4~12代细胞用于实验。分为5组:对照(control)组、缺氧(hyp)组、抑制剂(SB203580)组、给药(Tau)组、抑制剂+给药(SB+Tau)组,Tau的给药浓度为100 mmol·L^(-1),p38抑制剂SB203580浓度为20μmol·L^(-1),给药时间为12h。MTT检测不同浓度Tau对PAECs的抑制。使用Western blot及Real-time PCR方法检测p38通路蛋白及炎症因子ICAM-1,VCAM-1的表达情况。使用免疫荧光检测p38核位移情况。MTT结果显示随着Tau浓度增加,对PAECs增殖抑制增强。Western blot和Real-time PCR结果显示在蛋白水平及基因水平Tau都抑制ICAM-1,VCAM-1的表达。Western blot的结果和免疫荧光的结果均显示Tau可以抑制p38蛋白的活化。Tau可能通过p38 MAPK通路抑制由缺氧引起的牛肺主动脉内皮细胞中ICAM-1,VCAM-1的表达。
To investigate the effect of taurine（Tau） on ICAM-1, VCAM-1 by p-p38 pathway in bovine pulmonary artery endo- thelial cells（PAECs） and explore its mechanism of action. Generation 4-12 cells in primary cultures of PAECs were used in ex- periments and divided into five groups： control group, hypoxia （hyp） group, inhibitor （ 5B203580 ） group, treatment （Tau） group, and treatment ＋ inhibitor（ SB ＋ Tau） group. The concentration of Tau： 100 mmol . L-1 ; p38 inhibitor SB203580 ： 20μmol·L-1 ; and the treatment time was 12 h. MTT assay was used to detect the inhibitory effect of different concentrations of Tau on PAECs. Western blot and Real-time PCR method were used to detect the p38 pathway proteins and ICAM-1, VCAM-1 expression levels. Immunofluorescence was used to investigate p38 nuclear displacement situation. The results of MTT showed that the inhibitory effect was gradually increased with increasing concentrations of Tau. Western blot and RT-PCR revealed that the protein and mRNA expression levels of ICAM-1 , VCAM-1 were reduced by Tau. Western blot and immunofluorescence showed Tau can inhibit p38 activation. Tau may decrease the expression levels of VCAM-1 and ICAM-1 in endothelial cells in- duced by hypoxia through MAPK p38 pathway.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第12期2350-2354,共5页
China Journal of Chinese Materia Medica
基金
黑龙江省自然基金重点项目(160672)
