摘要
The pathogenesis of equine Osteoarthritis（OA） is more complex, and the disease in the early stage is not easy to be found, therefore, the early diagnosis and treatment are very important. Based on this, this experiment established OA model induced by equine, aimed to study the changes of contents of Matrix Metalloproteinases-3（MMP-3）, Matrix Metalloproteinases-13（MMP-13）, Aggrecanase（ADAMTS-5）, Hyaluronic Acid（HA） and Osteocalcin（OCN） in synovial fluid, and establish rapid diagnostic technique for the equine OA. Thirteen Mongolian equines were used in these induction studies. Equines were randomly divided into two groups： the experimental group contained eight equines and the control group contained five equines. The experimental group was to build the equine osteoarthritis model. The induction was done through Intra-articular（IA） injection of 2 m L Amphotericin-B in equines’ left carpal joints. The equine of the control group was injected into 2 m L physiological saline in equines’ left carpal joints. Synovial fluid was collected every week until the 9th week. The contents of MMP-3, MMP-13, ADAMTS-5, HA and OCN in synovial fluid were evaluated by using ELISA kits. Equine OA model, compared with the control group, starting from the 1st to the 2nd week after induction model, the content of MMP-3, MMP-13, ADAMTS-5, HA and OCN tended to increase, but there was no significant increase, from the 2nd to the 3rd week they significantly increased（p〈0.05） and kept increasing trend until the 9th week. In OA model, MMP-3, MMP-13, ADAMTS-5, HA and OCN showed a rising trend in joint fluid, which would accelerate the cartilage, subchondral bone degradation and metabolism of these proteases increased, and ADAMTS-5 and HA in the early stage increased significantly.
The pathogenesis of equine Osteoarthritis（OA） is more complex, and the disease in the early stage is not easy to be found, therefore, the early diagnosis and treatment are very important. Based on this, this experiment established OA model induced by equine, aimed to study the changes of contents of Matrix Metalloproteinases-3（MMP-3）, Matrix Metalloproteinases-13（MMP-13）, Aggrecanase（ADAMTS-5）, Hyaluronic Acid（HA） and Osteocalcin（OCN） in synovial fluid, and establish rapid diagnostic technique for the equine OA. Thirteen Mongolian equines were used in these induction studies. Equines were randomly divided into two groups： the experimental group contained eight equines and the control group contained five equines. The experimental group was to build the equine osteoarthritis model. The induction was done through Intra-articular（IA） injection of 2 m L Amphotericin-B in equines’ left carpal joints. The equine of the control group was injected into 2 m L physiological saline in equines’ left carpal joints. Synovial fluid was collected every week until the 9th week. The contents of MMP-3, MMP-13, ADAMTS-5, HA and OCN in synovial fluid were evaluated by using ELISA kits. Equine OA model, compared with the control group, starting from the 1st to the 2nd week after induction model, the content of MMP-3, MMP-13, ADAMTS-5, HA and OCN tended to increase, but there was no significant increase, from the 2nd to the 3rd week they significantly increased（p〈0.05） and kept increasing trend until the 9th week. In OA model, MMP-3, MMP-13, ADAMTS-5, HA and OCN showed a rising trend in joint fluid, which would accelerate the cartilage, subchondral bone degradation and metabolism of these proteases increased, and ADAMTS-5 and HA in the early stage increased significantly.
基金
Supported by the National Science and Technology Support Program of China(2012BAD46B02-03)
