摘要
目的探讨SH2B1基因多态性与妊娠糖尿病患者胰岛素抵抗的关系。方法选取164例妊娠期糖尿病(GDM)患者和130例糖耐量正常孕妇。采用限制性片段多态性聚合酶链反应检测SH2B1基因型,并且分析相关临床资料。结果 GDM组SH2B1(rs4788102)基因GA型与GG型比较,差异有统计学意义[OR=1.531,(95%CI:1.093,2.374)P=0.04],A等位基因频率与G等位基因频率比较,差异有统计学意义[OR=1.436,(95%CI:1.091,1.972)P=0.01]。GDM组GA+AA基因型与GG基因型比较,差异有统计学意义[OR=1.699,(95%CI:1.185,2.479)P=0.02]。GDM组中GA+AA基因型体重指数、三酰甘油、瘦素及稳态模型评估胰岛素抵抗指数与GG基因型比较,差异有统计学意义(P<0.05)。经Logistic回归分析显示,GA+AA基因型为稳态模型法测定胰岛素抵抗指数升高的危险因素。结论 SH2B1(rs4788102)基因多态性可能是GDM的一个易感位点,监测孕妇该基因位点,可以用于GDM发生风险的预测。
Objective To investigate the relationship between SH2B1 gene polymorphism and insulin resis- tance in patients with gestational diabetes mellitus （GDM）. Methods Totally 164 GDM patients and 130 normal pregnant women with impaired glucose tolerance were selected. The SH2B1 genotype was detected by poly- merase chain reaction-restriction fragment length polymorphism and then the clinical data were analyzed. Results There was significant difference between the GA and GG genotypes of SH2B1 （rs4788102） in the GDM group [C}R= 1.531, （95% CI： 1.093, 2.374）, P= 0.04]. There was significant difference in the A allele frequency and the G allele frequency in the GDM group [C}R = 1.436, （95% CI： 1.091, 1.972）, P= 0.01]. In the GDM group, the GA and AA genotypes were significantly different from the GG genotype [C}R = 1.699, （95% CI： 1.185, 2.479）, P= 0.02]. BMI, TG, leptin, and HOMA-IR were significantly different between the GDM patients with AA or GA genotype and those with GG genotype （P 〈 0.05）. Logistic regression analysis showed that GA and AA genotypes were the risk factors for HOMA-IR elevation. Conclusions SH2B1 （rs4788102） gene polymorphism may be a susceptible locus of GDM, which could be used to predict the risk of GDM in pregnant women.
出处
《中国现代医学杂志》
CAS
北大核心
2017年第12期55-59,共5页
China Journal of Modern Medicine
