摘要
应用PCR产物直接测序法分析漆树种群nrDNA(核糖体DNA)ITS序列和cpDNA序列(matK、rbcL、psb AtrnH)的碱基差异,初步研究两套植物基因组的变异速率。结果表明:nrDNA ITS序列共有518 bp,有变异位点15处,变异位点百分率为2.9%,(G+C)含量为61.8%。cpDNA序列合并后长度1 907 bp,有变异位点20处,变异位点百分率为1.05%,(G+C)含量为36.1%。通过对ITS序列核糖型(Ribotype)和叶绿体序列单倍型(Haploype)进行分析发现,秦巴山区漆树区域性分布明显,不同区域拥有自己独特的单倍型,漆树居群历史近期没有扩张。nrDNA ITS序列较叶绿体序列进化较快,变异速率较快。nrDNA ITS序列及叶绿体matK、psbA-trnH适合漆树的亲缘地理学研究。
We analyzed the differences between nrDNAITS and cpDNA sequences m loxtcoaenaron vernicifluum by PCR direct sequencing. The length of nrDNA ITS sequence of T. vernicifluum was 518 bp, of which 15 were variable sites with a percentage of 2.9%, and the（ G ＋ C） percentage content was 61.8%. The length of cpDNA sequence of T. vernicifluum was 1 907 bp, of which 20 was variable site with a percentage of 1.05%, and the （G ＋ C） content was 36. 1%. By ITS sequence Ribotype and chloroplast sequence Haplotypes, the regional distribution of sumac in Qinba Mountains is obvious and different area has its unique haplotype. There is no expansion in the recent history of sumac populations. The evolution of T. vernicifluum nrDNA ITS sequence is faster than that of chloroplast sequence, and the mutation rate is faster. The nrDNA ITS sequence and cpDNA sequence （matK and psbA-trnH） are fit to the phylogeographic study for this species.
出处
《植物研究》
CAS
CSCD
北大核心
2017年第4期579-586,共8页
Bulletin of Botanical Research
基金
国家科技支撑计划项目(漆树特色种质挖掘与创新利用[2013BAD01B06-7])~~
关键词
漆树
ITS序列
叶绿体DNA序列
Toxicodendron vernlcifluum
ITS sequence
cpDNA sequenct
