摘要
目的 :观察survivin蛋白在氟中毒SD大鼠牙胚发育过程中的表达,探讨氟对survivin蛋白表达的影响,进一步探讨氟斑牙的发病机制。方法:取妊娠10 d孕鼠45只,随机分为对照组、实验1组和实验2组,饮水中含氟浓度分别为0、50、150 mg/L。每组分别在E18.5 d(妊娠18.5 d)、E20.5 d、P1.5 d(出生后1.5 d)、P3.5 d、P5.5 d时取材、固定、包埋,选择P1.5 d标本做H-E染色。应用免疫组织化学SABC法对每组切片染色。利用Motic Med 6.0数码医学图像分析系统(A)进行图像采集。采用SPSS 13.0软件包对数据进行单因素方差分析。结果:在牙胚发育的不同时期,各组survivin蛋白的表达均呈"M"形变化趋势。E18.5d组F=1.050,P>0.05;E20.5 d组F=2.232,P>0.05,各组间差异无显著性;P1.5 d组F=3.538,P<0.05,P3.5 d组F=3.820,P<0.05,P5.5 d组F=5.096,P<0.05。实验组与对照组survivin的表达有显著差异。新生鼠各组经SNK法比较,对照组与实验1组之间的survivin表达差异无显著性(P>0.05);对照组与实验2组之间的差异有显著性(P<0.05)。结论:氟使新生鼠survivin蛋白表达量减少,且氟浓度越高,这种抑制作用越明显,可能是氟牙症发生的一条途径。
PURPOSE： This study was to investigate the expression of survivin in dental germ development of SD rats with fluorosis, and explore the effects of fluoride on survivin expression and the pathogenic mechanism of dental fluorosis. METHODS： Forty-five SD rats （pregnant for 10 days） were randomly divided into control group, experimental group 1 and group 2. Drinking water with fluoride concentration of 0, 50, 150 mg/L was provided for rats accordingly. The samples were collected at E18.5th day, E20.5th day, P1.5th day, P3.5th day and P5.5th day, then the samples of P1.5th day were selected for H-E staining. SABC method was used for immunohistochemical analysis of samples in each group. The images were acquired by Motic Med 6.0 digital medical image analysis system, and the data were analyzed for ANOVA with SPSS 13.0 software package. RESULTS： Survivin expression fluctuated and exhibited ＂M＂ shape （rose first and fell later） in each group.There was no significant difference at E18.5th day （F=1.050, P〉0.05） and E20.5th day F=2.232, P〉0.05） between each group. There were significant differences at P1.5th day （F=3.538, P〈0.05）, P 3.5th day F=3.820, P〈0.05） and P5.5th day （F=5.096, P〈0.05） between the control and experimental groups. The postnatal rats in each group were evaluated by SNK method for surviving expression. There was no significant difference between control group and experimental group 1 （P〉0.05）. However, there was significant difference between control group and experimental group 2 （P〈0.05）. CONCLUSIONS： The results suggest that fluoride can decrease the expression of survivin in the postnatal rats with higher fluoride concentration, which may be the mechanism in the development of dental fluorosis.
作者
钟翠翠
田剑刚
黄瑞哲
齐红
邓转云
ZHONG Cui-cui TIAN Jian- gang HUANG Rui-zhe QI Hong DENG Zhuan-yun(Department of Stomatalogy, People＇s Hospital of Yanggu City. Yanggu 252300, Shandong Province Department of Preventive Dentistry, Stomatological Hospital of Medical College of Xi＇an Jiaotoag Uaiversity, Key Laboratory of Shaanxi Province for Craniofoial Precision Medicine Research. Xi＇an 710004, Shaanxi Province, China)
出处
《上海口腔医学》
CAS
CSCD
2017年第3期254-257,共4页
Shanghai Journal of Stomatology
基金
陕西省科技攻关项目(2003K10-G22)
