摘要
目的建立高效液相色谱法同时测定黑参中稀有人参皂苷20(S)-Rg_3、20(R)-Rg_3、Rk1、Rg_54种有效成分的含量。方法采用COSMOSIL C_(18)-PAQ(4.6 mm×250 mm,5μm)色谱柱,流动相为水-乙腈梯度洗脱,检测波长203 nm,柱温30℃,流速1.0 m L·min^(-1),进样体积10μL。结果稀有皂苷20(S)-Rg_3、20(R)-Rg_3、Rk1、Rg_5得到良好的分离效果,分别在0.051~0.256 mg·m L^(-1)(r=0.999 9)、0.009~0.280 mg·mL^(-1)(r=0.999 7)、0.051~0.303 mg·m L^(-1)(r=0.999 9)和0.093~0.279 mg·m L^(-1)(r=0.999 9)内与色谱峰面积呈良好的线性关系。平均加样回收率分别为103.9%、99.2%、97.0%和100.6%,RSD分别为0.71%、0.73%、1.97%和0.57%。结论本实验所建立的黑参中皂苷含量测定方法准确、简单、可靠、重复性好,可用于黑参药材中4种主要化学成分含量的测定,其结果可为其质量控制、合理用药及进一步研究开发提供参考。
OBJECTIVE To establish an HPLC method for determining four kinds of rare saponins, ie, 20 （S） -Rg3,20 （R） -Rg3, Rkz, and Rgsin black ginseng METHODS COSMOSIL C18-PAQ （4. 6 mm×250 mm, 5 μm） column was used and temperature was maintained at 30℃. Gradient elution was conducted using mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 203 nm. The injected sample volume was 10μL RESULTS Good resolution was achieved for the four rare sapenins in the ranges of 0. 051 -0. 256 （r =0. 999 9） ,0. 009 -0. 280（r =0. 999 7） ,0. 051 -0. 303（r =0. 999 9） and 0. 093-0. 279 mg·mL^-1 （r =0. 999 9） for 20（S）-Rg3,20（R）-Rg3, Rk1, and Rg5, respectively. The corresponding average recovery rates were 103.9%, 99. 2%, 97.0%, and 100. 6%, and the standard deviations were 0. 71%, 0. 73%, 1.97%, and 0. 57%, respec- tively. CONCLUSION The method is accurate, simple, reliable and reproducible for the determination of saponins in black ginseng. The determination result can be used as a reference for the rational medication, quality control, and further study of black ginseng.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2017年第12期1069-1072,共4页
Chinese Pharmaceutical Journal
基金
吉林省自然科学基金资助项目(20160101017JC)
吉林省教育厅资助项目(吉教科合字[2016]第197号)
吉林省重点科技攻关资助项目(20170204023NY)
