摘要
目的研究LAIR-1受体在LPS诱导RAW264.7细胞发生炎症反应中表达规律的变化。方法以LPS分别刺激RAW264.7细胞6、12、24和48 h。采用Greiss法测定NO的含量,ELISA法测定TNF-α、IL-6的含量,Western blot法检测LAIR-1受体的表达。NF-κB抑制剂PDTC(50μmol/L)预处理1 h后,检测LPS刺激24 h后LAIR-1受体的表达。结果随着LPS刺激RAW264.7细胞时间的延长,NO、TNF-α和IL-6分泌量显著增加,LAIR-1受体表达量逐渐降低;经过PDTC预处理后,与LPS处理组比较,LAIR-1受体表达量上调。结论 LAIR-1受体在LPS诱导RAW264.7细胞发生炎症反应过程中表达量逐渐下调,抑制NF-κB通路其表达量上调,LAIR-1受体可能与炎症反应密切相关。
The purpose of the present study is to investigate the expression changes of LAIR-1 receptor inRAW264.7 cells induced by LPS. The cells were stimulated with LPS for 0, 6, 12, 24 or 48 h. Then the content ofNO was assayed by Greiss method, the concentrations of TNF-α and IL-6 in the culture supernatant were analyzedby ELISA, and the expression of LAIR-1 receptor was determined with Western blot. Also, the cells werepre-treated with NF-κB inhibitor PDTC（50 μmol/L） for 1 h and were then cultured with LPS for 24 h followed bydetection of LAIR-1 receptor. Data showed that expression of LAIR-1 decreased gradually when the production ofNO, TNF-α and IL-6 increased in RAW264.7 cells stimulated by LPS for different time. When the cellspre-treated with PDTC, the expression of LAIR-1 was higher than that of LPS alone group. Taken together, LAIR-1would declined rapidly in LPS-activated macrophages, and up-regulated when NF-κB pathway inhibited,suggesting LAIR-1 is closely related with the development of inflammation.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2017年第7期575-579,共5页
Immunological Journal
基金
山东省博士基金(BS2014YY049)
烟台市科技计划(2014ZH092)
科研启动基金(BY2013KYQD09)
国家自然科学基金(81503339)
