摘要
目的通过观察顺铂损伤小鼠肾小管上皮细胞后Bcl-2、Bax及caspase-3的表达以及白藜芦醇干预后其表达的变化,探讨白藜芦醇对顺铂肾小管上皮细胞损伤的保护机制。方法体外培养小鼠近曲小管上皮细胞(mProx)并分为4组:对照组(Con)、顺铂25μM组(CIS)、白藜芦醇+顺铂25μM组(RES+CIS),(100μmol白藜芦醇预处理3 h后加入25μM顺铂作用24 h)、白藜芦醇组(RES)。台盼蓝拒染方法测定细胞活力,Tunel法检测细胞凋亡,Western blot法检测Bcl-2、Bax及caspase-3的表达变化。结果与对照组相比,顺铂组细胞活力下降(P<0.05),细胞凋亡增加(P<0.05),Bax及caspase-3的蛋白表达增强(P<0.05),Bcl-2蛋白表达下降(P<0.05);与CP组比较,RES+CIS组细胞活力明显增加(P<0.05),细胞凋亡减少(P<0.05),Bax及caspase-3的表达下降(P<0.05),Bcl-2的表达上升(P<0.05)。结论白藜芦醇能有效提高小鼠近曲小管上皮细胞存活率,抑制细胞凋亡,其机制可能与其通过下调caspase-3、Bax及上调Bcl-2有关。
Objective To observe the expression of Bcl-2 ,Bax and caspase-3 in mouse tubular epithelial cells after cisplatin-induced cytotoxicity, before and after the treatment with resveratrol, and to explore the underlying mechanism of resveratrol against cisplatin-induced cytotoxicity. Methods Mouse proximal tubular cells（mProx） cultured in vitro were divided into 4 groups, controls （ C ）, cisplatin alone at 25 μM （ CIS）, cisplatin 25μM plus resveratrol 100 μM （ CV ＋ RES）, and resveratrol alone 100 p,M （RES） ,25 μM cisplain, pretreated with 100 μM resveratrol for 3 hours, cells were stimulated for 24 hours. Cell proliferation activity was detected by Trypan Blue Assay, analysis of cell apoptosis by Tunel Assay, Bcl-2, Bax and caspase-3 were detected by using Westemblot. Results Compared with the control group, cellular viability in the cisplatin group was decreased （ P 〈 0. 05 ）, cell apoptosis was increased （ P 〈 0.05 ） , the expression of Bax, caspase- 3 were increased（ P 〈 0.05 ）and the expression of Bcl-2 was decreased in the cisplatin group ;resveratrol can increase cellular viability, restrain cell apoptosis （ P 〈 0. 05 ）, lower the activity of Bax and caspase-3 （ P 〈 0.05 ） and increase the activity of Bax （ P 〈 0.05 ）. Conclusion Resveratrol can effectively increase cell viability, minimize apoptosis in mouse proximal tubular cells. The mechanism may be via down-regulating the expression of Bax, caspase-3 and increasing the expression of Bcl-2.
出处
《中华全科医学》
2017年第8期1334-1336,1432,共4页
Chinese Journal of General Practice
