摘要
目的观察电针干预后,对功能性消化不良(functional dyspepsia,FD)大鼠胃窦ghrelin、哺乳动物雷帕霉素靶蛋白(m a m m a l i a n target of rapamycin,mTOR)、磷酸化mTOR(phosphorylation mTOR,p-mTOR)表达的影响.方法将36只大鼠按随机数字表随机分为空白组及造模组,其中空白组10只,雌雄各半.将除空白组外的26只大鼠进行造模,将造模成功的20只大鼠再随机分为模型组和电针组,每组各10只,雌雄各半.造模方法均采用夹尾刺激法(每天2次,14 d)配合不规则饮食(每逢周一、三、五禁食,余日正常饮食)制备FD大鼠模型.造模成功后,电针组给予电针足三里进行干预,1次/d,共10 d.干预结束后对3组大鼠进行解剖取胃部组织,用Western blot分别检测各组大鼠胃窦ghrelin、mTOR、p-mTOR的表达水平.结果与空白组相比,模型组大鼠胃窦ghrelin水平显著降低(0.15450±0.050145 vs 0.31550±0.83885,P<0.01),电针干预后,FD大鼠胃窦组织中ghrelin水平明显升高(与模型组相比,0.23930±0.53918 vs 0.15450±0.050145,P<0.05).与空白组相比,模型组大鼠mTOR、p-mTOR蛋白表达水平显著升高(mTOR/円-actin:0.77190±0.070404vs 0.59520±0.064768;p-mTOR/円-actin:0.792±0.098164 vs 0.48620±0.101248,均P<0.01);与模型组相比,电针干预组胃窦mTOR、p-mTOR表达水平明降低(mTOR/円-actin:0.669±0.043726 vs 0.77190±0.070404;p-mTOR/円-actin:0.6346±0.073699 vs 0.77190±0.070404,均P<0.05).结论电针干预后,能够显著增加FD大鼠胃窦ghrelin表达水平,以改善胃肠动力,同时对mTOR、p-mTOR表达水平同样存在着显著影响,提示mTOR及其磷酸化可能在电针促进ghrelin的分泌的过程中起重要的作用,从而调节胃肠道的活动.
AIM To observe the effect of electro-acupuncture （EA） on the expression of ghrelin, mammalian target of rapamycin （mTOR） and phosphorylated mTOR （p-mTOR） in the gastric antrum of rats with functional dyspepsia （FD）. METHODS Thirty rats were randomly divided into a blank group （n = 10）, a model group （n = 10）, and an EA group （n = 10）. Experimental FD was induced in rats of the model group and EA group by tail clamp stimulation twice a day for 14 d and giving an irregular diet （fasting every other day, with free access to water）. After successful induction of FD, the EA group was given electro-acupuncture at Zusanli once a day for 10 d. The expression of ghrelin, mTOR and p-mTOR in the gastric antrum was detected by Western blot. RESULTS Compared with the blank group, the level of ghrelin in the gastric antrum was significantly decreased in the model group （0.15450 ±0.050145 vs 0.31550 ± 0.83885, P 〈 0.01）, but EA intervention significantly reduced the level of ghrelin in the gastric antrum of rats with FD compared with the model group （0.23930± 0.53918 vs 0.15450 ± 0.050145, P 〈 0.05）. Compared with the blank group, the relative expression levels of mTOR and p-mTOR in the model group were increased （0.77190 ±0.070404 vs 0.59520 ± 0.064768, 0.792 ± 0.098164 vs 0.48620 ± 0.101248, P 〈 0.05 for both）. Compared with the model group, the relative expression levels of mTOR and p-mTOR in the antrum group were significantly lower in the EA group （0.669 ± 0.043726 vs 0.77190 ±0.070404, 0.6346 ±0.073699 vs 0.77190± 0.070404, P 〈 0.05 for both）. CONCLUSION EA intervention can increase the expression of ghrelin in gastric antrum of FD rats, suggesting that EA intervention may increase the gastrointestinal moffiity. EA intervention can also decrease the expression of mTOR and p-mTOR, suggesting that mTOR and its phosphorylation may play an important role in the process that EA promotes the secretion of ghrelin, thereby regulating gastrointestinal activity.
作者
康朝霞
张红星
徐派的
王计雨
韩永丽
Zhao-Xia Kang Pai-Di Xu Ji-Yu Wang Yong-Li Hart Hong-Xing Zhang(College of Acu-moxibustion and Fractures, Hubei University of Chinese Medicine, Wuhan 430065, Hubei Province, China Wuhan Integrated TCM ＆ Western Medicine Hospital, Wuhan 430022, Hubei Province, China)
出处
《世界华人消化杂志》
CAS
2017年第17期1553-1557,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.8147379
湖北中医药大学"青苗计划"基金资助项目
No.2016ZZX011~~
