摘要
目的探讨中、重度脂血分别对Sysmex XE-2100及XN-9000血液分析仪主要指标的影响。方法取20例无溶血、黄疸、脂血标本,将每例均分为3管,每例均取一管组成一组。第1组为对照组,其余两组标本分别去除50μL和100μL血浆后,加入等体积脂肪乳剂制备成脂血标本,用Siemens Advia2400全自动生化分析仪测定三酰甘油(TG),根据TG水平不同,按脂血严重程度分级第2组TG 10.1~20.0mmol/L设为中度脂血组,第3组TG>20.0mmol/L设为重度脂血组。所有标本分别用Sysmex XE-2100及XN-9000全自动血液分析仪进行检测,将不同程度脂血标本测定结果分别与对照组对应标本进行比较。结果对于XE-2100,中度脂血时血红蛋白(Hb)、平均红细胞血红蛋白含量(MCH)和平均红细胞血红蛋白浓度(MCHC)水平与对照组相比,差异有统计学意义(P<0.05),重度脂血时白细胞(WBC)计数、血细胞比容(HCT)、Hb、平均红细胞体积(MCV)、MCH和MCHC水平与对照组相比,差异均有统计学意义(P<0.05);而对于XN-9000,无论脂血严重程度,Hb、HCT、MCV、MCH和MCHC水平与对照组相比,差异均有统计学意义(P<0.05)。XE-2100的WBC-BASO结果均较WBC-DIFF明显升高(P<0.05),且中、重度脂血标本的WBC-BASO结果均较对照组明显升高(P<0.05),而XN-9000的WBC-WNR与WBC-DIFF结果无差异,且中、重度脂血标本的WBC-WNR结果与对照组差异无统计学意义(P>0.05)。结论无论机型及脂血严重程度,WBC(DIFF通道)、红细胞和血小板计数及WBC五分类的结果均未受影响;Hb、HCT、MCV、MCH和MCHC的结果均受影响;XN-9000的抗脂血干扰能力优于XE-2100(BASO通道)。
Objective To investigate the influence of moderate and severe lipemia on the main indicators of the Sysmex XE-2100 and XN-9000 hematology analyzers. Methods Twenty samples without hemolysis,jaundice and lipemia were selected. Each sample was equally divided into 3 tubes. Each sample took one tube for making up one group. The first group served as the control group, the samples in the other two groups respectively removed 50,100μL plasma, then the isovolumetric lipid emulsion was added to prepare the lipemia sample. Then the TG level was detected by the Siemens Advia2400 automatic biochemical analyzer. According to the different TG level,the samples were graded according to the lipemia severity;the second group（TG 10.1-20.0 mmol/L） was set as the moderate lipemia group;the third group （TG〉20.0 mmol/L） was set as the severe lipemia group. All samples were de- tected by using the XE-2100 and XN-9000 hematology analyzers. Then the detected results of different degrees of lipemia samples were respectively compared with the results of cori＇esponding samples in the control group. Results For XE-2100,the levels of Hb, MCH and MCHC in the moderate lipemia group had statistical difference（P〈0.05）, the WBC count and levels of Hb, HCT, MCV, MCH and MCHC in the severe lipemia group had statistical difference（P〈0.05） ;but for XN-9000, no matter lipemia severity,the levels of Hb, HCT, MCV, MCH and MCHC had statistical difference（P〈0.05）. The WBC-BASO results of XE-2100 were signifi- cantly increased compared with WBC-DIFF（P〈0. 05） ,moreover the WBC-BASO results of moderate and severe lipemia samples were significantly increased compared with the control group（P〈0.05）, but the WBC-WNR and WBC-DIFF results in XN-9000 had no difference,moreover the WBC-WNR results of moderate and severe lipemia samples had no statistical difference compared with those in the control group（P〉0.05）. Conclusion No matter the machine types and lipemia severity,the results of WBC（DIFF channel）, RBC,PLT count and WBC fivepart differentiation all are not affected; the results of Hb, HCT, MCV, MCH and MCHC all are affected;the anti-lipemia interference ability of the XN-9000 is superior to the XE-2100（BASO channel）.
出处
《检验医学与临床》
CAS
2017年第12期1735-1737,1740,共4页
Laboratory Medicine and Clinic
关键词
脂血
血液分析仪
血液分析
lipemia
hematology analyzer
blood analysis
