摘要
目的:研究干扰素调节因子(IRFs)对颈动脉粥样硬化患者斑块性质、T细胞亚群分化的影响。方法:选择在我院诊断为颈动脉粥样硬化的68例患者作为AS组、同期体检的80例健康志愿者作为对照组。采集外周血单个核细胞并检测IRFs的表达量,采集血清并检测细胞因子及斑块稳定性指标的含量。结果:AS组患者外周血单个核细胞中IRF1、IRF8的mRNA表达量较对照组显著升高,IRF2、IRF4的mRNA表达量较对照组显著降低;AS组患者血清中IFN-γ、IL-2、IL-17、IL-22、MCP-1、oxLDL、MMP9、Caspase-3的含量显著高于对照组且与IRF1、IRF8的mRNA表达量呈正相关,与IRF2、IRF4的mRNA表达量呈负相关;IL-4、IL-10、TGF-β的含量显著低于对照组且与IRF1、IRF8的mRNA表达量呈负相关,与IRF2、IRF4的mRNA表达量呈正相关。结论:颈动脉粥样硬化患者体内IRF1、IRF8的高表达以及IRF2、IRF4的低表达会降低斑块稳定性、影响Th1/Th2/Th17/Treg的分化。
Objective： To study the effect of interferon regulatory factors （IRFs） on the plaque nature and T cell subset differentiation in patients with carotid atherosclerosis.Methods： A total of 68 patients who were diagnosed with carotid artery atherosclerosis in Puren Hospital of Wuhan City between June 2014 and December 2016 were selected as AS group, and 80 healthy volunteers who received physical examination during the same period were selected as control group.Peripheral blood mononuclear cells were collected to detect the expression of IRFs, and serum was collected to detect the levels of cytokines and plaque stability indicators.Results： IRF1 and IRF8 mRNA expression in peripheral blood mononuclear cells of AS group were significantly higher than those of control group while IRF2 and IRF4 mRNA expression were significantly lower than those of control group;serum IFN-γ, IL-2, IL-17, IL-22, MCP-1, ox-LDL, MMP9 and Caspase-3 levels of AS group were significantly higher than those of control group, positively correlated with IRF1 and IRF8 mRNA expression, and negatively correlated with IRF2 and IRF4 mRNA expression;IL-4, IL-10 and TGF-β levels were significantly lower than those of control group, negatively correlated with IRF1 and IRF8 mRNA expression, and positively correlated with RF2 and IRF4 mRNA expression.Conclusions： The high expression of IRF1 and IRF8 and the low expression of IRF2 and IRF4 in patients with carotid atherosclerosis will reduce the plaque stability and affect the Th1/Th2/Th17/Treg differentiation.
出处
《海南医学院学报》
CAS
2017年第10期1326-1329,共4页
Journal of Hainan Medical University
基金
湖北省自然基金项目(2010CDB8701)~~
