摘要
目的探究胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)对早期激素性股骨头坏死(steroid-induced avascular necrosis of the femoral head,SANFH)的保护作用。方法采用改良技术建立早期激素性股骨头坏死兔模型,随机分成实验组(n=12)和对照组(n=12)。实验组给予髋周臀大肌注射GDNF(8.0μg/只),对照组相同部位注射等体积生理盐水,干预1周。分别于第5、10、15、20天进行股骨头多排螺旋CT、显微CT及苏木素-伊红(H&E)染色检测。结果影像学观察示,GDNF注射后第15天开始,实验组股骨头骨小梁逐渐丰富、密集,间距变窄,骨皮质厚度及密度增加;对照组股骨头骨小梁分布稀疏,间距增宽,部分断裂,骨质密度减低。第15、20天,实验组与对照组股骨头骨矿物质密度、组织骨矿物质密度及骨体积分数,比较差异具有统计学意义(P<0.05)。组织学观察示,实验组股骨头内骨小梁粗大,骨细胞分布广泛,细胞核着色深,脂肪细胞分布较少;对照组骨小梁分布稀疏,形态不完整,部分断裂,骨细胞空陷窝和脂肪细胞增多,骨细胞和造血细胞减少。第15、20天,实验组与对照组股骨头骨细胞空陷窝率分别为:13.3±3.2%、10.6±2.4%和71.0±7.5%、78.6±5.3%,两组比较,实验组骨细胞空陷窝率显著减低(P<0.05)。结论 GDNF可通过抑制骨细胞坏死,保持骨小梁的完整性。
Objective The aim of this study was to explore the protective effects of glial cell line-derived neurotrophic factor（GDNF） on steroid-induced avascular necrosis of the femoral head（SANFH） in rabbits. Methods The rabbit model of ANFH was established by injection of low-dose lipopolysaccharide and high-dose glucocorticoid. Twenty-four New Zealand white rabbits were randomly divided into experimental and control groups. Each rabbit in the experimental group received eight microgram of GDNF intramuscular injection at gluteus maximus for one week,and rabbits in the control group were injected with normal saline of the same volume. Changes in the femoral head were examined using multi-slice spiral computed tomography（CT）,microcomputed tomography（micro-CT） and hematoxylin eosin（HE） staining at 5,10,15 and 20 days after modeling. Results The imagological diagnosis showed that from the 15 th day after the injection,trabecular bone of the femoral head in the experimental group had increased trabecular number and density and reduced separation,and the cortex and density of bone increased,whereas in the control group the trabeculae became sparse,thinner and broken,and the density of bone decreased. Bone mineral density（BMD）,tissue mineral density（TMD） and bone volume/total volume（BV/TV） in the experimental group were significantly higher than those of the control group at day 15 and 20（P〈0. 05）. Histological observation indicated that in the experimental groupthe trabeculae of the femoral head was thick,there were less adipocytes,and osteocytes in the bone lacunae were widely distributed and had normal shapes. In the control group,the trabeculae became thinner and broken,number of bone and hematopoietic cells decreased,and adipocytes and bone empty lacunae could obviously be seen. At day 15 and 20,the percentage of empty lacunae in the femoral head was 13. 3 ± 3. 2% and 10. 6 ± 2. 4% in the experimental group,which was lower than the 71. 0 ± 7. 5% and 78. 6± 5. 3% in the control group（P〈0. 05）. Conclusion GDNF can inhibit the necrosis of bone cells and maintain the structural integrity of bone trabeculae.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2017年第6期701-706,共6页
Chinese Journal of Osteoporosis
基金
国家临床重点专科建设项目([2013]544)
四川省教育厅基金资助项目(15ZB0243)
关键词
激素性股骨头坏死
胶质细胞源性神经营养因子
显微CT
骨密度
Glial cell line derived neurotrophic factor
Steroid induced avascular necrosis of the femoral head
Micro computed tomography
Bone mineral density
