摘要
目的目前鲜有专门针对稀有鮈鲫这种新型实验动物进行组织切片制作方法的研究,故我们对此进行了实践、摸索以建立一完整的组织学切片制作流程供参考交流。方法对正常生活状态下的健康稀有鮈鲫采用Bouin`s固定液固定,并用脱钙液软化骨骼后进行修块,组织块经梯度酒精脱水、松油醇透明之后,按照标准流程制成石蜡切片,HE染色后封片用显微镜观察。结果切片主要采取横断面和矢状面作为观察方位,并使用显微拍摄装备摄取了结构分明的各系统组织脏器的组织学照片。结论本文所述的方法经过多次探索和实践,制得的组织切片镜检结果清晰,质量稳定,可作为以稀有鮈鲫等小型硬骨鱼为实验动物的切片制作提供一些可行的建议。
Objective Gobiocypris rarus, a freshwater fish with high prolificacy, distributes in Sichuan Province. In order to became a laboratory animal, the fish has been investigated its geographical distribution, morphology, habita, ontogenesis, reproduction, growth, taxonomy, semitiviqto ecological factors, karyotype and so on by Chinese Academy of Science since 1990s. However, there was few histological background of Gobiocypris rarus until now. Here we introduce a practical way to make sections of Gobiocypris rarus. Method The healthy individuals were fixed in the Bouin＇ s fluid, washed with running water slightly, and put into decalcifying fluid. After finishing decalcification, the fish was cut into several small pieces, the specimens were dehydrated and infiltrated, respectively, and then waxed with paraffin. The specimens in solid paraffin were allowed to cut into serial slices. The slices were dyed by Hematoxylin and Eosin （H＆E） staining according to the routine protocol. Transverse and sagittal section pictures were collected by a optical microscope. Result The sections were scaned under microscope and the pictures of different kinds of organs and tissues were taken. Those high quality pictures can set up the histological background of Gobiocypris rarus. Conclusion Histology can detect features of disease which are difficult to recognize from clinical symptoms. The histological background of Gobiocypris rarus tissues and organs is the foundation of pathology knowledge. The method of making sections of Gobiocypris rarus may contribute to the research of aquatic animal.
出处
《实验动物科学》
2017年第2期31-34,共4页
Laboratory Animal Science
基金
国家科技支撑计划
课题编号:No.2015BAI07B02和No.2011BAI15B01-41
关键词
稀有鮈鲫
组织学
切片
HE染色
Gobiocypris rarus
histology
section
HE stainin
