摘要
目的建立反相高效液相色谱测定强力枇杷露中磷酸可待因的方法。方法采用Eclipse XDB-C_(18)柱(250 mm×4.6mm,5μm),乙腈-0.035 mol/L醋酸钠溶液(冰醋酸调pH=3.5)(25∶75)为流动相,体积流量为1.0 mL/min,检测波长为240 nm,柱温为40℃。结果磷酸可待因在2.024~40.24μg/mL线性良好,回归方程为y=8.18×10~5x+12.2(r=0.999 2);平均加样回收率为98.2%,RSD为1.0%(n=6)。结论该方法快速准确,灵敏度高,用于强力枇杷露中盐酸可待因含量测定,控制药品质量。
Objective To establish the determination method of Codeine phosphate in Qiangli Pipa Syrup by RP-HPLC.Methods Using XDB-C18 Eclipse column （250 mm ＆#215; 4.6 mm,5 ＆#236;m）,acetonitrile：0.035 mol/L sodium acetate solution （glacial acetic acid to adjust pH =3.5） （25：75） as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength is 240 nm,the column temperature is 40.Results the linear range of codeine phosphate in 2.024-40.24 ＆#236;g/mL was goody =8.18 ＆#215; 105x ＋ 12.2 （r =0.999 2）;the average recovery rate was 98.2% and RSD was 1.0% （n =6）.Conclusion This method is simple,rapid,accurate,and reliable,with high sensitivity,and could be applied to determination of Qiangli Pipa Syrup codeine hydrochloride content and controlment of the drug quality.
出处
《药物评价研究》
CAS
2017年第5期672-674,共3页
Drug Evaluation Research
关键词
强力枇杷露
盐酸可待因
固相萃取
高效液相色谱法
Qiangli Pipa Syrup
codeine hydrochloride
solid phase extraction
high performance liquid chromatography
