摘要
为了解茶树Cs MSD在干旱胁迫下的表达规律,采用RT-PCR和RACE技术,对茶树干旱胁迫响应相关重要基因锰超氧化物歧化酶基因Cs MSD进行克隆和生物信息学分析,并采用实时荧光定量PCR技术,对其在不同程度干旱胁迫下的表达规律进行分析。结果显示,茶树Cs MSD核苷酸序列全长1013bp,开放阅读框长693bp,共编码230个氨基酸;Cs MSD蛋白分子量为25.47k D,理论等电点p I=6.38,存在9个磷酸化位点,亚细胞定位于线粒体中,且蛋白质序列中具有MSD特征结构域;q PCR分析干旱胁迫下茶树叶片中Cs MSD的表达变化情况发现,Cs MSD在干旱胁迫下的相对表达量与对照组相比显著上调。研究认为铁观音茶树Cs MSD在应对干旱胁迫中发挥着重要的作用。
Abstract：The Manganese Superoxide Dismutase Gene,which plays important roles in response to drought stress,was cloned from Camellia sinensis cv. Tieguanyin. The gene sequence （ GenBank accession number, KP189419 ）was found to be 1013bp, containing a 693bp open reading frame which encodes 230 amino acids. The deduced protein molecular weight was 25.47 kD and its theoretical isoelectric point was 6.38. The bioinformatics revealed that the protein did have 9 phosphorylation sites,and that its subcellular cells located in mitochondria. The protein sequences had conserved functional domains related to manganese superoxide dismutase. QPCR analysis indicated that the expression level of CsMSD,when the tea trees was under varied drought stresses,was significantly higher than the control group,which suggested that CsMSD might play important roles during the processes of tea plant responding to the drought stresses.
出处
《贺州学院学报》
2017年第2期149-156,共8页
Journal of Hezhou University
基金
福建省2015年重大科技专项项目(2015NZ0002-1)
