摘要
目的鉴定分离自江苏省先天性畸胎的弓形虫虫株(KS株)和HIV-弓形虫共感染患者刚地弓形虫的基因型。方法抽提江苏省先天性畸胎弓形虫分离株(KS株)速殖子和1例HIV-弓形虫共感染患者的全血DNA,选择11个基因位点,采用聚合酶链式反应-限制性片段长度多态性分析(PCR-RFLP)进行基因型鉴定。结果自江苏省先天性畸胎分离的弓形虫虫株和1例HIV-弓形虫共感染患者的全血DNA样本中均获得完整分型条带,经鉴定均为弓形虫基因型Ⅰ型。结论Ⅰ型刚地弓形虫可能是江苏地区引起妊娠异常结局妇女和HIV-弓形虫共感染患者的优势基因型虫株。
Objective To identify the genotype of Toxoplasma gondii isolated strains from a congenital teras（KS strain）and an HIV-Toxoplasma co-infected patient in Jiangsu Province. Methods T. gondii DNA of tachyzoites of a isolate from a congeni-tal teras（KS strain）and blood DNA of an HIV-Toxoplasma co-infected patient in Jiangsu Province were extracted,and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）. Results The complete bands were obtained from the congenital teras（KS strain）and HIV-Toxoplasma co-infected patient in Jiangsu Province,and identified as T. gondii gene type Ⅰ. Conclusion T. gondii gene type Ⅰ may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV-Toxoplasma co-infected patients in Jiangsu Prov-ince.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2017年第3期329-333,共5页
Chinese Journal of Schistosomiasis Control
基金
"十一五"国家科技支撑计划重点项目(2010BAD04B02)
教育部创新团队发展计划(IRT0978)
江苏省人兽共患病学重点实验室开放课题(I1507)
江苏省高校优势学科建设工程资助项目
