摘要
目的采用高效液相色谱-串联质谱(HPLC-MS/MS)法建立保健品中阿伐那非和氟班色林的检测方法。方法采用Agilent Eclipse-plus C_(18)(2.1 mm×100 mm,1.8μm)色谱柱,流动相为乙腈-0.1%乙酸(含20 mmol·L^(-1)乙酸铵)溶液(60∶40)。质谱条件为电喷雾离子源,以多重反应监测方式进行检测。结果阿伐那非和氟班色林在2~20 ng·mL^(-1)范围内与峰面积均呈良好线性关系(r2>0.99),加样回收率为94.6%~110.0%,精密度RSD<5.0%,检出限分别为0.21和0.42μg·kg^(-1)。结论该方法测定快速准确,灵敏度高,专属性强,可用于保健品中阿伐那非和氟班色林的检测。
Objective To develop a method for detection of avanafil and flibanserin adulterated in health food by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. Methods The separation and analysis were performed on an Agilent Eclipse Plus C18 column（2.1 mm×100 mm,1.8 μm）,with a mobile phase of acetonitrile and 0.1%acetic acid（containing 20 mmol·L^-1 ammonium acetate）（60：40）.Electrospray ionization（ESI） source was applied and operated in positive mode.Multiple reaction monitoring（MRM） mode was used to quantify avanafil and flibanserin. Results The assay linearity of avanafil and flibanserin were confirmed in the range of 2—20 ng·mL^-1（r^2〉0.99）.The extraction recoveries varied from 94.6% to 110.0%,and the precision of RSD was 〈5.0%.The limits of detection were 0.21 and 0.42 μg·kg-1 for avanafil and flibanserin,respectively. Conclusion The method was specific,sensitive and accurate.Therefore,it can be used to detect avanafil and flibanserin which were illegally added in health food.
出处
《医药导报》
CAS
2017年第7期783-785,共3页
Herald of Medicine
