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寻常型银屑病患者AMSCs的分离培养和免疫调控功能鉴定 被引量:4

Isolating culture of adipose mesenchymal stem cells in psoriasis vulgaris patients and differentiation into immune regulation function
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摘要 目的对比寻常型银屑病患者和健康人来源的脂肪间充质干细胞(AMSCs)的表型、周期、成脂和成骨分化能力及免疫调控功能差异,探索患者AMSCs在银屑病发病机制中的作用。方法体外分离培养AMSCs,用流式细胞计量术检测AMSCs的细胞表型和周期;用油红O和碱性磷酶染色鉴定AMSCs的成脂和成骨分化能力;用PCR和ELISA检测AMSCs表达抑炎或促炎因子;通过基因表达谱芯片筛选两组AMSCs的免疫差异表达基因。结果健康人和银屑病患者AMSCs均高表达CD29、CD44及CD73和低表达CD45、CD31及HLADR。而且,都可分化成骨细胞和成脂细胞,但银屑病组AMSCs增殖趋缓(P<0.05),成脂分化能力较强。银屑病组较对照组AMSCs表达或分泌的抑炎因子IL-10、IDO及TGF-β等下降(P<0.05),而TNF-α及IFN-γ等促炎细胞因子增强(P<0.05)。银屑病组AMSCs的JAK-STAT通路与对照组比明显下调(P<0.05)。结论寻常型银屑病患者AMSCs较正常人的增殖和成脂分化能力出现异常,免疫抑炎调控能力减弱,推测可能与JAK-STAT免疫相关通路下调有关。 Objective To study the differences of the biological characteristics and immune regulation function of adipose mesenchymal stem cells (AMSCs)from psoriasis patients and healthy people. Methods AMSCs were iso- lated and cultured from human psoriatic and healthy adipose tissue, the phenotypes and cell cycle of AMSCs taken from three generation were detected by flow eytometry. Alkaline phosphate enzyme staining and oil red o staining were used respectively to identify their adipogenic and osteogenic capacity. Next, the levels of inflamma- tion antimicrobial proinflammatory factor were detected by PCR and ELISA. Then gene expression profile of AMSCs were screen by gene expression profile chip, as so to bolting the the gene array related with immunology gene.Resuits There was no significant change in cell morphology, and cell surface markers were expressed high for CD29,CD44,CD73, while lower for CD31, CD45 and HLA-DR. AMSCs of psoriasis patients and healthy people both had the ability of adipogenic and osteogenic differentiation. But the cell cycle showed the third generation AM- SCs proliferation rates were slower than that of normal control, as compared with healthy controls, adipogenic differ- entiation ability was stronger. What'more, the level of inflammatory cytokines in psoriasis group was lower than that in controls such aslL-10,IDO ,TGF-β, on the contrary the levels of proinflammatory factor in psoriasis group were higher than that in controls, such as TNF-α, IFN-β. In addition, gene chip results suggested that psoriasis group AMSCs had obvious expression differences on JAK-STAT pathway with healthy controls. Conclusions Compared with the control, there are significant differences in patients AMSCs proliferation and adipogenic differentiation abil- ity, immune inflammation suppression control ability is weaken, this phenomenon may be associated with JAK-STAT immune pathways related to downgrade.
出处 《基础医学与临床》 CSCD 2017年第7期975-981,共7页 Basic and Clinical Medicine
基金 国家自然科学基金(81573984)
关键词 寻常型银屑病 脂肪间充质干细胞 增殖 成脂和成骨能力 免疫调控 psoriasis vulgaris adipose mesenchymal stem cells proliferation adipogenic and osteogenic capacity immune regulation function
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  • 1Michalis Mastri,Huey Lin,Techung Lee.WJSC 6^(th) Anniversary Special Issues(2):Mesenchymal stem cells Enhancing the efficacy of mesenchymal stem cell therapy[J].World Journal of Stem Cells,2014,6(2):82-93. 被引量:16
  • 2Nickoloff BJ.Cracking the cytokine code in psoriasis[J],Na- ture Med,2007,13(3):242-244.
  • 3Spaggiari GM,Capobianco A,Becchetti S,et al.Mesenchy- mal stem cell-natural killer cell interactions:evidence that acti- vated NK cells are capable of killing MSCs,whereas MSCs can inhibit IL-2-induced NK-cell proliferation[J].Blood,2006,107(4):1484-1490.
  • 4Liu Y,Han ZP,Zhang SS,et al.Effects of inflammatory fac- tors on mesenchymal stem cells and their role in the promotion of tumor angiogenesis in colon cancer[J].J Biol Chem,2011, 286(28):25007-25015.
  • 5Zhang K,Liu R,Yin G, et al.Differential cytokine secretion of cultured bone marrow stromal cells from patients with pso- riasis and healthy volunteers[J].Eur J Dermatol,2010,20(1):49-53.
  • 6Majumdar MK,Keane-Moore M,Buyaner D,et al.Charac- terization and functionality of cell surface molecules on human mesenchymal stem cells[J],J Biomed Sci,2003,10(2):228-241.
  • 7Nemeth K,Keane-Myers A,Brown JM,et al.Bone marrow stromal cells use TGF-β to suppress allergic responses in a mouse model of ragweed-induced asthma[J],PNAS,2010,107(12):5652-5657.
  • 8Ren G,Zhang L,Zhao X,et al.Mesenchymal stem cell-medi- ated immunosuppression occurs via concerted action of chemo- kines and nitric oxide[J],Cell Stem Cell,2008,2(2):141-150.
  • 9Nie Y,Lau C,Lie A,et al.Defective phenotype of mesenchy- mal stem cells in patients with systemic lupus erythematosus[J],Lupus,2010,19(7):850-859.
  • 10Huiming Sheng Ying Wang Yuqing Jin Qiuyu Zhang Yan Zhang Li Wang Baihua Shen ShuoYin Wei Liu Lei Cui Ningli Li.A critical role of IFNγ in priming MSC-mediated suppression of T cell proliferation through up-regulation of BT-H1[J].Cell Research,2008,18(8):846-857. 被引量:55

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