摘要
背景:补肾活血通络胶囊是多年的临床验方,在治疗膝关节骨性关节炎效果明显,但是其疗效机制一直未明确。目的:探讨补肾活血通络胶囊对SD大鼠膝关节骨性关节炎滑膜细胞miR-27a的调控作用。方法:将采用Hulth法成功建立膝关节骨性关节炎模型的120只SD大鼠随机分为空白组、低剂量组、中剂量组、高剂量组。空白组大鼠根据体质量予以75 mg/kg生理盐水灌胃,低剂量组、中剂量组、高剂量组大鼠依据体质量予按34,75,140 mg/kg补肾活血通络胶囊水溶剂灌胃,2次/d,15 d为1疗程,连续灌胃2个疗程,每个疗程间间隔2 d。4组大鼠在灌胃1疗程、2个疗程后分批次取得大鼠膝关节滑膜及软骨,运用实时荧光定量PCR技术测定各组大鼠膝关节滑膜及软骨细胞miR-27a含量的表达。结果与结论:(1)大体观察可见补肾活血通络胶囊干预后的大鼠膝关节骨性关节炎模型的滑膜和软骨退变程度要轻于未经补肾活血通络胶囊干预的大鼠;(2)实时荧光定量PCR显示空白组、低剂量组、中剂量组、高剂量组滑膜及软骨miRNA-27a的表达量呈升高趋势,以高剂量最高,组间对比差异有显著性意义(P<0.05);(3)结果说明,补肾活血通络胶囊可抑制大鼠膝关节骨性关节炎模型滑膜及软骨细胞miRNA-27a表达量的降低,进而减缓大鼠膝关节骨性关节炎的退变,起到保护膝关节的作用。
BACKGROUND： Bushen Huoxue Tongluo Capsule, a well-known traditional prescription, exhibits remarkable treatment outcomes in knee osteoarthritis, but the underlying mechanism is not fully understood. OBJECTIVE： To explore the regulatory effect of Bushen Huoxue Tongluo Capsule on miR-27a of synovial cells in Sprague-Dawley rats with knee osteoarthritis. METHODS： The model of knee osteoarthritis was established in 120 Sprague-Dawley rats using Hulth method, and then these rat models were randomized into four groups, followed by given75 mg/kg normal saline （blank control group）, 34 （low-dose group）, 75 （medium-dose group） and 140 （high-dose group） mg/kg Bushen Huoxue Tongluo Capsule aqueous solvent via gavage according to body mass, respectively, twice daily, for consecutive 2 courses （15 days as a course） with 2 days in between. The synovium and cartilages were removed after each course, and the expression of miR-27a in the knee joint synovium and chondrocytes was determined by real-time quantitative PCR. RESULTS AND CONCLUSION： Gross observation found that the degeneration of synovium and cartilage in the Bushen Huoxue Tongluo Capsule groups was milder than that in the blank control group. Real-time PCR revealed that there was an increasing trend in the expression of miRNA-27a in the synovium and cartilage in each group, especially in the high-dose group, and there was a significant difference between groups （P 〈 0.05）. Our findings show that Bushen Huoxue Tongluo Capsule can suppress the reduced expression of miRNA-27a in the synovium and chondrocytes in rats with knee osteoarthritis, further alleviating knee degeneration and protecting the knee from damage.
作者
孙志涛
赖居易
何升华
王业广
王建
冯华龙
黄飞强
Sun Zhi-tao Lai Ju-yi He Sheng-hua Wang Ye-guang Wang Jian Feng Hua-long Huang Fei-qiang(Third Orthopedic Ward, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518000, Guangdong Province, China Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2017年第16期2484-2488,共5页
Chinese Journal of Tissue Engineering Research
基金
深圳市卫人委项目(201402080)~~
