摘要
背景:六味地黄丸作为补益肝肾名方,临床应用其治疗腰痛已取得良好的效果,而有关六味地黄丸治疗肾虚型腰痛的作用机制尚不明确。目的:观察六味地黄丸对兔椎间盘体外退变模型细胞外基质组分的影响,探讨六味地黄丸防治椎间盘退变的疗效。方法:将20只新西兰兔带终板的L1-L6椎间盘100个随机分为空白对照组、肿瘤坏死因子α组、p38-JNK/SAPK阻断组、六味地黄丸组、肿瘤坏死因子α+六味地黄丸组,每组20个。肿瘤坏死因子α组培养液中含10 mg/L肿瘤坏死因子α2μL,p38-JNK/SAPK阻断组培养液中含p38MAPK特异性阻断剂SB203580,JNK特异性阻断剂SP600125各20μmol/L 10μL,六味地黄丸组培养液中含体积分数为10%六味地黄丸血清,肿瘤坏死因子α+六味地黄丸组含10 mg/L肿瘤坏死因子α2μL和体积分数为10%六味地黄丸血清,分别于培养的第2,4,8,14天收集标本待测。结果与结论:在培养基中加入肿瘤坏死因子α能明显上调Ⅰ型胶原mRNA和蛋白的表达,下调糖胺多糖含量、硫酸软骨素/硫酸角质素比值、透明质酸含量,蛋白多糖mRNA和蛋白的表达、Ⅱ型胶原mRNA和蛋白的表达(P<0.05),六味地黄丸含药血清可部分对抗肿瘤坏死因子α所导致的改变,提示六味地黄丸可在一定程度上延缓了椎间盘退变。
BACKGROUND： Liuwei Dihuang Pills （LWPs）, a famous Chinese prescription for replenishing the liver and kidney, have achieved good effect in the treatment of backache. However, the mechanism underlying LWPs treating backache with kidney deficiency is unclear. OBJECTIVE： To observe the effect of LWPs on extracellular matrix components of rabbit intervertebral disc degeneration models in vitro, and to explore the effect of LWPs in the prevention and treatment of intervertebral disc degeneration.METHODS： Totally 100 L1-6 intervertebral discs with endplate removed from 20 New Zealand rabbits were randomly divided into control, tumor necrosis factor α （TNF-α）, p38-JNK/SAPK blocked, LWPs, and combination groups （n=20 per group）. 2 μL of 10 mg/L TNF-α, 10 μL of 20 μmol/L SB203580 （specific inhibitor of p38MAPK） and SP600125 （inhibitor of JNK）, 10% LWPs serum, 10% LWPs combined with 2 μL of 10 mg/L TNF-α were added into the medium of the corresponding group. Samples were collected at 2, 4, 6 and 14 days of culture. RESULTS AND CONCLUSION： The addition of TNF-α significantly upregulated the mRNA and protein expression levels of collagen type I, while significantly downregulated the levels of glycosaminoglycan and hyaluronic acid, chondroitin sulfate/keratan sulfate ratio, as well as the protein and mRNA expression levels of proteoglycan and collagen type Ⅱ （P 〈 0.05）. LWPs could partly reverse the changes caused by TNF-α, indicating that LWPs can alleviate intervertebral disc degeneration to a certain extent.
作者
徐无忌
杨康
刘思华
Xu Wu-ji Yang Kang Liu Si-hua(Department of Spine Surgery, the Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410005, Hunan Province, China Department of Traumatology and Orthopaedics, Yueyang Hospital of Traditional Chinese Medicine, Yueyang 414000, Hunan Province, China Second Department of Orthopaedics, Taojiang People＇s Hospital, Taojiang 413400, Hunan Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2017年第16期2495-2501,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然青年科学基金课题(81302993)
湖南中医药科技计划重点项目(201317)~~
