摘要
目的对从广州白云国际机场口岸入境的一例自苏里南归国发热人员进行寨卡病毒实验室检测。方法采集发热人员的血液和唾液样本,同时使用两种寨卡病毒实时荧光RT-PCR试剂进行寨卡病毒核酸检测。RT-PCR扩增寨卡病毒部分基因片段,并进行序列测定和同源性比较。基于扩增片段构建系统进化树,分析输入性病例的来源。结果实时荧光RT-PCR结果显示,该病例血清样本寨卡病毒核酸弱阳性、唾液样本寨卡病毒核酸阳性。RT-PCR扩增出预期大小约1.5Kb片段,测序结果表明该片段与巴西寨卡病毒株(GenBank号KX197250)相应序列的同源性为99%。系统进化树显示该病毒属亚洲谱系。结论根据患者流行病学史、临床表现和病例标本核酸检测情况,确诊该病例为广东首例从苏里南地区输入性寨卡病例。
We detected Zika virus（ZIKV）in a febrile case returning from Suriname and entry China from Guangzhou Baiyun International Airport Port.Serum and saliva samples were collected from a suspected case returning from Suriname.We detected ZIKV RNA using real-time fluorescence RT-PCR methods by both in-house reagent and commercial detection kits.RT-PCR detection was carried out with saliva sample and sequence analysis was performed.Phylogenetic tree was constructed to analyze the source of imported cases.Real-time fluorescent RT-PCR result showed that saliva was detected ZIKV RNA positive while for serum was weakly positive.A specific 1 500 bp fragment in size was amplified with saliva sample by RT-PCR.Sequence analysis showed 99% homologous to the corresponding sequence of Brazil ZIKV（GenBank No.KX197250）.Phylogenetic tree indicated it was located on African lineage.According to the epidemiological investigation results,clinical manifestations and nucleic acid detection of case,the suspected case was confirmed to infect Zika virus,being the first case from Suriname into Guangdong Province.
作者
梁洁怡
戴俊
黎东红
师永霞
黄吉城
袁帅
郑夔
李小波
张显光
宋卫
吴惠明
LIANG Jie-yi DAI Jun LI Dong-hong SHI Yong-xia HUANG Ji-cheng YUAN Shuai ZHENG Kui LI Xiao-bo ZHANG Xian-guang SONG Wei WU Hui-ming(Inspection and Quarantine Technical Center, Ouangdong Entry-exit Inspection and Quarantine Bureau, Guangzhou 510700, China Guangdong Entry-exit Inspection and Quarantine Bureau, Guangzhou 510623, China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2017年第6期522-525,共4页
Chinese Journal of Zoonoses
基金
广东省科技计划项目(No.2016A020247001)
广东检验检疫局科技计划项目(No.2016GDK58)联合资助~~
关键词
寨卡病毒
实时荧光RT-PCR
核酸检测
进化分析
Zika virus
real-time fluorescent RT-PCR
nucleic acid detection
phylogenetic analysis
