摘要
为探讨涡流空化强化载药壳聚糖微球的制备效果,研究传统离子凝胶法制备壳聚糖载药微球的最佳工艺,了解空化强化制备的载药微球的体外释放规律,在单因素试验的基础上,通过四因素三水平的响应面分析法研究了壳聚糖质量浓度、甲基异噻唑啉酮(MIT)浓度、三聚磷酸钠(TPP)质量浓度、搅拌转速对壳聚糖抗菌微球包封率的影响.结果表明,响应面法优化的最佳工艺为:壳聚糖质量浓度3.5 g/L,MIT浓度0.50 mmol/L,TPP质量浓度2.5 g/L,搅拌转速1 500 r/min,搅拌时间20 min,载药微球包封率为37.64%;在此基础上,涡流空化20 min,涡流空化出口压力0.3MPa时,微球的包封率达50.33%,比传统法优化后制备微球的包封率高了12.69%;涡流空化制备的载药微球在体外释放60 h后,MIT的累积释放量达78.79%.与传统离子凝胶制备方法相比,涡流空化能有效提高壳聚糖微球载药的包封率.
In order to study the enhancement preparation of drug-loaded chitosan microspheres by swirling cavitation, the optimal conditions of preparation of drug-loaded ehitosan microspheres by conventional ioniccrosslinking method, and the release of drug-loaded chitosan microspheres by swirling cavitation in vitro were investigated. Based on the single factor experiment and four-factor and three-level response surface methodology, the effects of chitosan concentration, methylisothiazolinone （MIT） concentration, tripolyphosphate （TPP） concentration, stirring speed on entrapment efficiency of ehitosan microspheres were studied. The results showed that the optimum technological conditions were as follows： ehitosan concentration 3.5 g/L, MIT concentration 0.50 mmol/L, TPP concentration 2.5 g/L, stirring speed 1 500 dmin, stirring time 20 min, and the entrapment efficiency could be up to 37.64%. On the basis of the optimal technological conditions, the swirling cavitation was introduced to enhance preparation of drug-loaded ehitosan microspheres, and the cavitation time was kept for 20 rain, the outlet pressure of swirling cavitation was kept at 0.3 MPa, then the entrapment efficiency could be up to 50.33%, which increased by 12.69% than that obtained by the optimized conventional ionic-crosslinking method. The accumulation of drug releasein vitro for drug-loaded ehitosan microspheres prepared by swirling cavitation could be up to 78.79% after 60 h. Compared with the conventional ionic-erosslinking method, it is concluded that the enhancement preparation of drug-loaded ehitosan microspheres by swirling cavitation is an effective method.
出处
《广西科技大学学报》
2017年第3期52-60,共9页
Journal of Guangxi University of Science and Technology
基金
国家自然科学基金项目(31160472)
广西高等学校高水平创新团队及卓越学者计划项目(桂教人[2014]7号)
广西高校糖资源加工重点实验室开放基金项目(2016TZYKF04)
广西科技大学博士基金项目(校科博Z04)资助
关键词
涡流空化
壳聚糖微球
包封率
swirling cavitation
chitosan microsphere
encapsulation efficiency
