摘要
目的研究灯盏花素(breviscapine,Bre)对大鼠动脉粥样硬化的保护作用。方法采用高脂饲料喂养结合腹腔注射维生素D3(VD3)的方法建立动脉粥样硬化大鼠模型,分别于造模过程同步灌胃给予Bre 6、12、24 mg·(kg·d)^(-1)和辛伐他汀SV 1.8 mg·(kg·d)^(-1),共12周,并设模型组和正常对照组。计算各组大鼠心脏指数(cardiac index,CI),通过HE染色观察主动脉形态变化并进行病变分级,生化分析法测定血清血脂指标,比色法测定血浆炎症细胞因子含量水平和血清中抗氧化酶活性及丙二醛(MDA)含量。结果与模型组比较发现Bre 12、24 mg·(kg·d)^(-1)组动脉粥样硬化大鼠CI显著降低(P<0.05);Bre各组大鼠主动脉病变呈不同程度改善,Bre 24 mg·(kg·d)^(-1)组大鼠主动脉病变分级水平显著降低(P<0.05);Bre 12、24 mg·(kg·d)^(-1)组血清血脂指标TC、TG、LDL-C含量显著降低(P<0.05),血浆炎症细胞因子CRP、TNF-α、IL^(-1)β、IL-6含量明显降低(P<0.05),血清抗氧化酶SOD、CAT活性显著升高且MDA含量显著降低(P<0.05);Bre24 mg·(kg·d)^(-1)组血清血脂指标HDL-C含量和抗氧化酶GSH-Px活性均显著升高(P<0.05),血浆中炎症细胞因子IL^(-1)0含量显著升高(P<0.05)。结论 Bre对大鼠动脉粥样硬化具有一定的保护作用,其机制可能与Bre能够有效调节血脂、抑制炎症反应和降低氧化应激损伤有关。
Objective To investigate the protective effects and mechanism of breviscapine（ Bre） on atherosclerosis rats. Methods The atherosclerosis models were made by high-fat diet and intraperitoneal injection with VD3,and at the same time,the rats were treated with Bre [6,12,24 mg·（ kg·d）^-1]and simvastatin [SV,1. 8 mg·（ kg·d）^-1],course of the treatment was 12 weeks; the rats were divided into normal control group and model group. The cardiac index（ CI） was calculated; the histopathological changes and lesion classification of abdominal aorta was observed by HE staining; the serum lipids in serum were detected; the level of inflammatory cytokines in serum were determined; the activity of antioxidase and the content of MDA in serum were determined. Results Compared with model group,the CI of Bre [12,24 mg·（ kg·d）^-1]groups were significantly decreased（ P〈0. 05）; the histopathological changes were significantly improved; the level of TC,TG,LDL-C in serum of Bre [12,24 mg·（ kg·d）^-1]groups were significantly decreased（ P〈0. 05）; the level of CRP,TNF-α,IL^-1β,IL-6 in plasma were significantly decreased（ P〈0. 05）; the activity of SOD,CAT in serum were significantly increased while the content of MDA was significantly decreased（ P〈0. 05,P〈0. 05）; the level of HDL-C and the activity of GSH-Px in Bre 24 mg·（ kg·d）^-1group was significantly increased（ P〈0. 05）,the level of IL^-10 was significantly increased（ P〈0. 05）. Conclusion Bre has protective effects on athero-sclerosis rats,which perhaps related to its effects of reducing cholesterol,improving the anti-inflammatory and antioxidant capacity.
出处
《环球中医药》
CAS
2017年第6期669-673,共5页
Global Traditional Chinese Medicine
关键词
灯盏花素
动脉粥样硬化
血脂
炎症
氧化应激
Breviscapine
Atherosclerosis
Blood lipid
Inflammation
Oxidative stress
