摘要
为了实现农产品中农药残留含量快速分析,根据荧光光谱的基本原理研究了腐霉利的荧光特性,对腐霉利-蜂蜜混合体系进行了研究,分析荧光强度与蜂蜜中腐霉利含量之间的关系。采用稳态荧光光谱仪分别检测腐霉利溶液和蜂蜜溶液的荧光光谱,纯蜂蜜溶液荧光峰在342 nm,腐霉利溶液的荧光峰在390 nm,通过逐次向纯蜂蜜溶液中添加0.1 g/d L的腐霉利标准溶液,发现相对于纯蜂蜜,荧光峰位置发生红移,说明腐霉利与蜂蜜中某些物质发生了相互作用。采用Origin8.5数据分析软件对蜂蜜的荧光峰值处荧光峰强度与腐霉利含量线性拟合后,发现蜂蜜溶液中腐霉利的含量与对应的荧光强度有良好的线性关系,预测函数模型为I=188504.75x+384764.33,相关系数为0.99。经过相同实验条件下的实验验证,模型预测准确率在96.8%~100%之间。研究结果表明:荧光光谱法检测蜂蜜中腐霉利残留含量的方法是行之有效的,为其他农产品中腐霉利农药残留含量的检测提供了参考依据。
In order to offer the quick analysis of concentration of pesticides residues in agricultural products,a mixed procymidone-honey system was built and studied according to the principle of fluorescence spectrum. The relationship between the fluorescence intensity and the procymidone content in honey was also analyzed. With the aid of steady fluorescence spectrometer,the fluorescence spectra of procymidone solution and honey solution were detected respectively. The fluorescence emission peaks of pure honey and procymidone are found at about 342 nm and 390 nm. The fluorescence peak position has a red shift（from 342 nm to 390 nm） with respect to an addition of procymidone（0. 1 g/d L） into honey,indicating the interaction between procymindone and some substances in honey. By using software program of Origin8. 5 which is used to process and analyze data,the linear fitting is done of the fluorescence peak intensity of the honey and the content of procymindone. The results show that the prediction models between the fluorescence intensity and procymidone content in honey has a good linear relationship. The function model is I = 188504. 75 x ＋ 384764. 33,and the correlation coefficient is 0. 99. The experimental results under the same conditions show that the accuracy of the function model is 96. 8%-100%. The results can be concluded that the determination of procymidone is effective and reliable for the concentration testing of procymidone residues in other agricultural products.
出处
《发光学报》
EI
CAS
CSCD
北大核心
2017年第7期973-977,共5页
Chinese Journal of Luminescence
基金
国家自然科学基金(61475071)
教育部博士点(博导类)专项基金(20093218110024)资助项目~~
关键词
荧光光谱
蜂蜜
腐霉利
含量检测
fluorescence spectrum
honey
procymidone
concentration detection
