摘要
将1.00g·L^(-1) DNA溶液与1.00mmol·L^(-1)三氯化铁溶液混合制得DNA-Fe(Ⅲ)配合物溶液。取溶液20μL滴涂于经抛光的GCE表面,滴加0.50g·L^(-1) CTS溶液10μL,于20℃干燥22h制得DNA-Fe/CTS修饰的GCE电极。利用扫描电子显微镜对DNA-Fe/CTS BPICM的形貌进行了表征。采用循环伏安法和安培-时间曲线法研究该修饰电极的电化学特性及该电极对过氧化氢的电化学响应。结果表明,固定在聚合膜中的铁离子表现出较好的电化学活性,DNA-Fe/CTS/GCE对过氧化氢的还原反应具有较好的电催化活性。由此提出了一种新型生物相容性过氧化氢电化学传感器。该传感器的线性范围为0.01~2.0mmol·L^(-1),检出限(3S/N)为3μmol·L^(-1)。
Complex of DNA-Fe（Ⅲ） was prepared by mixing 1.00 g · L-1 DNA solution with 1.00 mmol · L-1 FeCla solution. A portion （20 μL） of the DNA-Fe（ Ⅲ ） solution was applied on the surface of the polished GCE, and then 10 μL of 0. 50 g · L-1 CTS solution were dropped onto the GCE to give DNA-Fe/CTS modified GCE electrode after drying at 20 ℃ for 22 h. Morphology of the modified electrode was characterized by SEM, and its electrochemical behavior and response to H2O2 were studied by cyclic voltammetry and the i-t curve. It was found that better electrochemical activity was exhibited by the Fe（ Ⅲ ） embedded in the DNA/CTS layer and that better electrocatalytic activity for reduction of H2O2 at the modified electrode of DNA-Fe/CTS/CK2E was observed. Based on these facts, a novel, bio-eompatible electrochemical hiosensor for H2O2 was proposed. Linearity range of this biosensor was found between 0. 01 and 2. 0 mmol· L^- 1 H2O2 with value of detection limit （3S/N） of 3μmol · L^- 1.
出处
《理化检验（化学分册）》
CSCD
北大核心
2017年第7期753-758,共6页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
辽宁省自然基金项目(2015020624)
辽宁省教育厅创新团队项目(LT2014007)
辽宁省教育科学"十二五"规划课题(JG11DB140)
辽宁科技大学优秀人才专项基金(201301)
辽宁科技大学研究生教育创新计划教育教学改革研究与实践项目(2014YJSCX07)
