摘要
目的通过图文结合的形式,详细介绍大鼠蛛网膜下腔出血模型的制作方法及过程;改进并创新模型,使其能更好的模拟临床动脉瘤破裂所致蛛网膜下腔出血。方法选取SD雄性大鼠,采用颈内动脉颅外段穿入颈内段法,建立SD雄性大鼠蛛网膜下腔出血模型,用呼吸、瞳孔、大小便及留置纤芯解剖大鼠头颈等方法对模型进行验证。通过观察死亡率和临床表现,探讨模型的可控性、可重复性。结果实现了刺破血管造成蛛网膜下腔出血,且纤芯到达的位置能准确的刺破所需要的血管。结论此法制作模型稳定,方法通俗易懂,操作精巧灵活,可重复性强,理解后短时间内就可造模成功,且成功造模所形成的蛛网膜下腔出血,能更好地模拟临床动脉瘤突然破裂出血,非常适用于蛛网膜下腔出血早期脑损害及其血管痉挛的研究。
Objective To report a newly developed method and procedure to establish a rat model of subarachnoid hemorrhage in detail,and to provide a better model simulating the clinical subarachnoid hemorrhage caused by a ruptured aneurysm for related research. Methods One hundred and twenty healthy SPF 2-3-month old male Sprague-Dawley rats were divided into 4 groups,30 rats in each group. The three experimental groups were sacrificed at 6,24 and 72 hours after modeling. Rat models of subarachnoid hemorrhage were established by inserting a fiber core in the internal carotidartery and piercing this artery. Successful establishment of the subarachnoid hemorrhage model was confirmed by observation of breathing, pupil, defecation, urination and inspection at autopsy dissection. The controllability and reproducibility of this model were verified by observation of clinical manifestation and explored by mortality analysis.Results Subarachnoid hemorrhage was successfully induced by fiber core piercing the internal carotid artery at the needed location. Conclusions This method of model preparation is stable and understandable. The operation is nimble,with a good reproducibility. This model can be successfully performed after a short time learning,well simulate the sudden hemorrhage caused by a ruptured aneurysm, and suitable for research on early brain injury and vasospasm after subarachnoid hemorrhage.
出处
《中国比较医学杂志》
北大核心
2017年第6期37-45,共9页
Chinese Journal of Comparative Medicine
基金
河北省省级重大医学项目(Zd2013093)
关键词
蛛网膜下腔出血
血管穿刺法
模型
大鼠
纤芯
Subarachnoid hemorrhage（SAH）
Aneurysm
Internal carotid artery
Surgery
Animal model
Rat
Fiber core
