摘要
目的评估Uf-1000i尿沉渣分析仪检测白细胞和细菌的基本性能及对尿路感染的筛查价值。方法收集304例疑是尿路感染做尿培养的患者的中段尿,用尿沉渣分析仪Uf-1000i检测尿白细胞(WBC)和细菌(BACT)。分析两项参数的精密度、线性和携带污染率。以尿细菌培养阳性为金标准,绘制尿白细胞和细菌计数的ROC曲线,并评价其诊断尿路感染的敏感度、特异度、假阳性率、假阴性率、阳性预期值、阴性预期值。结果 Uf-1000i检测白细胞和细菌的批内精密度、日间精密度、携带污染率和线性范围均符合厂家标准;Uf-1000i尿沉渣分析仪用于诊断尿路感染的阈值,为白细胞计数173.5个/μL和细菌计数513.5个/μL。若以白细胞或细菌任意一项阳性来判断尿路感染,敏感度、特异度、假阳性率、假阴性率、阳性预期值、阴性预期值分别为83.3%、73.0%、27.0%、16.7%、62.9%、88.8%。以白细胞和细菌双阳性来判断尿路感染,敏感度、特异度、假阳性率、假阴性率、阳性预期值、阴性预期值分别为54.6%、93.9%、6.1%、45.4%、83.1%、79.0%。结论 Uf-1000i尿沉渣分析仪检测白细胞和细菌的性能符合厂家标准,对尿路感染能早期筛查,便于临床医师决定是否需要培养及选择早期用药。
Objective To evaluate the performance of white blood cells（ WBC） and bacteria（ BACT） analyses in urinalysis analyzer Uf-1000 i and determine the potential application for urinary tract infection screening. Methods A total of 304 cases with suspected urine infection were collected. The performance including precision,linearity and carryover contamination rates in Uf-1000 i were determined. ROC curve was determined,and the sensitivity,specificity,false negative rates,expected positive rate and expected negative rate were evaluated compared with gold standard method-urine culture. Results Uf-1000 i showed good performance of within-run precision,intra-day precision,carryover,and linearity range for both WBC and BACT. The cutoff value based on ROC analysis for urinary infection using Uf-1000 i was further determined： 173.5 WBC/μL and 513.5 bacteria/μL. The sensitivity,specificity,false positive rate,false negative rate,positive predictive value,negative predictive for either WBC or BACT in Uf-1000 i were 83. 3%,73.0%,27.0%,16.7%,62. 9%,88. 8%,respectively. The sensitivity,specificity,false positive rate,false negative rate,positive predictive value,negative predictive for both WBC and BACT in Uf-1000 i were 54.6%,93.9%,6.1%,45.4%,83.1%,79.0%,respectively. Conclusion The performance of WBC and BACT analysis in Uf-1000 i fulfilled the requirement. Therefore,it was able to be applied as rapid screening equipment for clinical urinary infection,which facilitates identification of bacteria species and offers guidance of early drug administration during clinical practice.
出处
《东南国防医药》
2017年第3期234-238,共5页
Military Medical Journal of Southeast China
基金
南京市医学科技发展重点项目(ZKX13028)
关键词
尿路感染
白细胞
细菌
中段尿培养
ROC曲线
Urinary tract infection
White blood cells
Bacteria
Mid-stream urine culture
ROC curve
