摘要
目的:观察长春西汀注射液对脂多糖(lipopolysaccharide,LPS)诱导大鼠急性肺损伤(acute lung injury,ALI)的作用,并研究初步的作用机制。方法:雄性Wistar大鼠50只,随机分为正常对照组(control)、模型组(ALI组)以及长春西汀低剂量组、中剂量组和高剂量组,每组10只。正常对照组股静脉注射0.9%氯化钠注射液(5 m L/kg);模型组股静脉注射LPS 10 mg/kg;长春西汀低、中和高剂量组股静脉注射LPS 10 mg/kg,30 min后分别腹腔注射长春西汀注射液0.2 mg/kg、0.7 mg/kg和1.2 mg/kg。伊红染色观察肺部组织病理学切片,TUNEL法检测肺组织的细胞凋亡,分光光度法检测并计算肺组织髓过氧化物酶(MPO)活性,Western blot法检测肺组织中NF-κB、ICAM-1、VCAM-1、Bax与Bcl-2的蛋白水平。结果:与模型组相比,采用长春西汀给药后,明显减轻急性肺损伤的肺组织结构损伤与炎性细胞浸润,降低肺组织凋亡的细胞数与MPO活性,下调细胞中NF-κB、ICAM-1、VCAM-1与Bax的蛋白表达水平,上调Bcl-2的蛋白表达水平。结论:长春西汀注射液对急性肺损伤大鼠的肺组织具有保护作用,可能与降低肺组织中MPO活性,以及调控NF-κB、ICAM-1、VCAM-1、Bax与Bcl-2蛋白表达水平有关。
AIM： To observe the inhibitory effects of vinpocetine injection on lipopolysaccharide （L P S ） - in -duced acute lung injury （ ALI） in the rats and to explore the underlying mechanisms. METHODS： Male Wistar rats （n = 50） were randomly divided into 5 groups： control group, ALI model group, and low, medium and high doses of vinpocetine treatment groups. The rats in control group were injected with 0. 9% NaCl at 5 mL/kg through femoral vein. The rats in ALI model group received LPS at 10 mg/kg through femoral vein. After injected with LPS, the rats in vinpocetine treatment groups received vinpocetine at 0. 2 mg/kg, 0. 7 mg/kg or 1. 2 mg/kg via intraperitoneal injection. The pathological changes of the lung tissues were observed under microscope with HE staining. The cell apoptosis in the lung tissues was detected by TUNEL staining. Myeloperoxidase （MPO） activity was measured by the method of spectrophotometry. The protein expres-sion of NF-kB, ICAM-1, VCAM-1, Bax and Bcl-2 was determined by Western blot. RESULTS： Compared with ALI group, administration of vinpocetine significantly attenuated the structural injury of the lung and the infiltration of inflamma-tory cells. Moreover, vinpocetine decreased cell apoptosis and MPO activity in the lung tissues of ALI rats. In addition,the protein expression of NF-kB, ICAM-1, VCAM-1 and Bax was inhibited after vinpocetin treatment, whereas Bcl-2 expres-sion was increased. CONCLUSION： Vinpocetine attenuates LPS-lung injury by reducing MPO activity and regulating NF- kB, ICAM-1, VCAM-1, Bax and Bcl-2 protein expression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2017年第7期1278-1282,共5页
Chinese Journal of Pathophysiology
基金
广东省财政技术研究开发与推广应用项目(No.2060403)
关键词
长春西汀注射液
脂多糖
急性肺损伤
Vinpocetine injection
Lipopolysaccharide
Acute lung injury
