摘要
目的对采自中国南海山海绵Mycale sp.的化学成分进行研究。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等多种色谱学分离手段,对山海绵Mycale sp.的乙酸乙酯萃取层进行分离纯化;应用现代波谱技术,结合理化性质与文献报道对化合物进行结构鉴定;用Cell Counting Kit-8(CCK-8)法对化合物进行体外人乳腺癌细胞株MCF-7及人肺癌细胞株PC9细胞生长抑制活性进行测试。结果共分离得到10个化合物,分别鉴定为:环(脯-异亮)二肽[cyclo-(Pro-Ile)](1),环(脯-亮)二肽[cyclo-(Pro-Leu)](2),环(异亮-亮)二肽[cyclo-(Ile-Leu)](3),环(苯丙-脯)二肽[cyclo-(PhePro)](4),环(苯丙-缬)二肽[cyclo-(Phe-Val)](5),环(苯丙-亮)二肽[cyclo-(Phe-Leu)](6),环(苯丙-异亮)二肽[cyclo-(PheIle)](7),2′-deoxythymidine(8),胸腺嘧啶(thymine)(9),5-hydroxy-3,4-dimethy-5-pentyl-2(5H)-furanone(10)。经体外活性筛选发现,这些化合物对MCF-7及PC9未显示明显的生长抑制活性。结论化合物1、2、4、5、6、7和10均为首次从该属海绵中分离得到,本研究首次对化合物1~10的抗肿瘤活性进行了评价。
Objective To investigate the chemical constituents of marine sponge Mycale sp.collected from the South China Sea.Methods The ethyl acetate extract of the marine sponge Mycale sp.was separated and purified by repeated column chromatography on silica gel,Sephadex LH-20 and reversed-phase high-performance liquid chromatography(RP-HPLC).The structures of these compounds were identified by means of various modern spectroscopic techniques and comparison with their physicochemical properties to reported data.The tumor cell growth inhibitory activities of these compounds against human breast cancer cell lines MCF-7and human lung cancer cell lines PC9 were tested by Cell Counting Kit-8(CCK-8)method.Results Ten compounds were isolated and identified as cyclo-(Pro-Ile)(1),cyclo-(Pro-Leu)(2),cyclo-(Ile-Leu)(3),cyclo-(Phe-Pro)(4),cyclo-(Phe-Val)(5),cyclo-(Phe-Leu)(6),cyclo-(Phe-Ile)(7),2′-deoxythymidine(8),thymine(9),5-hydroxy-3,4-dimethyl-5-pentyl-2(5H)-furanone(10).These compounds showed weak tumor cell growth inhibitory activities toward cells MCF-7and PC9 in vitro.Conclusion Compounds 1,2,4,5,6,7 and 10 were isolated from the sponge Mycale sp.for the first time.It is the first time to report the antitumor activity evaluation for compounds 1~10.
出处
《药学实践杂志》
CAS
2017年第4期308-314,共7页
Journal of Pharmaceutical Practice
基金
国家自然科学基金(81373321)
