摘要
目的探讨醒脑静注射液预处理对七氟烷(sevoflurane)麻醉小鼠认知功能的影响及其潜在机制。方法用3%七氟烷持续麻醉6 h,模拟七氟烷麻醉的小鼠模型将动物随机分为对照组、模型组、醒脑静组,并于麻醉前2 h分别干预。监测麻醉过程中的血氧饱和度(SPO2)和心率;应用Morris水迷宫实验观察小鼠逃避潜伏期的变化;应用RT-PCR法和免疫印迹法检测大脑海马组织中脑源性神经营养因子(brain derived neurotropic factor,BDNF)的表达水平。结果监测显示:各组麻醉过程中的SPO2及心率均无统计学差异;Morris水迷宫显示:麻醉后模型组逃避潜伏期显著高于对照组(P<0.01),醒脑静组较模型组显著降低(P<0.05);RT-PCR结果显示:模型组BDNF的m RNA表达水平显著低于对照组(P<0.01),醒脑静组的表达水平较模型组显著升高(P<0.01);免疫印迹法结果与PT-PCR法一致。结论醒脑静预处理可改善七氟烷麻醉小鼠导致的认知功能障碍,且通过调节BDNF的表达发挥作用。
Objective To investigate the effect of Xingnaojing injection pretreatment on cognitive function and its poten-tial mechanism in sevoflurane anesthetized mouse. Methods Mice were anaesthetized with 3% sevoflurane for 6h, and pretreat-mented at 2 h before anesthesia. The model mice were randomly divided into the control group, model group and Xingnao-jing group. he oxygen saturation （SPO2） and heart rate during anesthesia were monitored. Morris water maze was used to ob-serve the changes of escape latency in mice, RT-PCR and Western blot test were used to detect the expression levels of brain derived neurotrophic factor （BDNF）. Results The results showed that there was no significant difference in SPO2 and heart rate during anesthesia. After sevoflurane exposure, the escape latency of model group was significantly higher than that of control group （P〈0.01）, and Xingnaojing group significantly decreased than model group （P〈0.05）. RT-PCR showed that the mRNA expression level of BDNF in model group was significantly lower than that in control group （P〈0.01）, and Xing-naojing group was significantly higher than model group （P〈0.01）. The results of Western blot analysis were in agreement with PT-PCR results. Conclusion Xingnaojing injection pretreatment could improve sevoflurane-induced cognitive impairment in mouse through regulating the expression of BDNF.
出处
《湖南中医药大学学报》
CAS
2017年第8期827-831,共5页
Journal of Hunan University of Chinese Medicine
基金
无锡市医院管理中心面上项目(YGZXM1502)
