摘要
目的:优化剪刀股中总皂苷的超声提取工艺,建立剪刀股中总皂苷的含量测定方法。方法:在单因素试验的基础上,采用Box-Behnken设计,结合响应面分析法,以剪刀股总皂苷的提取率为评价指标,采用分光光度法,测定剪刀股中总皂苷的含量,采用Design-Expert v8.0.6软件对实验结果进行拟合和建模,确定剪刀股总皂苷超声提取的最佳工艺。结果:超声法提取剪刀股中总皂苷的最佳工艺为乙醇浓度80%,液料比18∶1,温度71℃,超声时间15 min,超声功率200 W,在此条件下,剪刀股中总皂苷提取率为1.744%,RSD=0.65%(n=6)。结论:该方法操作简便、准确、灵敏、重复性好,可作为剪刀股总皂苷的提取及含量测定方法。
Objective：To optimize theextraction of total saponinsand establish a method for determination of total saponins inIxeris debilis.Method：Using the extraction yield of total saponins as index，spectrophotometric method was used to determinate the content of total saponins in I.debilis.On the basis of single factor experiments，Box-Behnken design and response surface were used to optimize theextraction of total saponins.And the extraction yield of the total saponins wasanalyzedusing quadratic regression modelby the software of Design-Expert v8.0.6.Result：The optimum extraction conditions were：ethanol concentration 80%，liquid to material ratio 18 mL·g^-1，temperature 71 ℃，extraction time 15 min and the ultrasonic power 200 w.Under these conditions，the yield of extracted total saponinscould be up to 1.744%，RSD=0.65%（n=6）.Conclusion：The method is simple，accurate，sensitive andreproducible，and can be used for extraction and content determination of total saponins from I.debilis.
作者
王威
孙晓丽
WANG Wei SUN Xiaoli(Pharmacy of The 3th Hospital of Zhangjiakou, Hebei, Zhangfiakou, 75000, China Women and Children Hospital of Yixian, Hebei, Baoding, 074200, China)
出处
《中国现代中药》
CAS
2017年第7期1021-1025,共5页
Modern Chinese Medicine
关键词
剪刀股
总皂苷
超声提取
含量测定
响应面法
Ixeris debilis A.Gray
total saponins
ultrasonic-assisted extraction
content determination
response surface methodology
