摘要
目的:研究Flavopiridol对胃肠道间质瘤细胞GIST882增殖、侵袭、凋亡及细胞周期的影响。方法:体外培养GIST882细胞至指数增长期,采用不同浓度的Flavopiridol对其进行干预,MTT法检测细胞增殖活性,Transwell小室实验检测细胞侵袭能力,流式细胞术检测凋亡及周期比例,western-blot检测CDK1,CDK2及caspase3蛋白的表达。结果:Flavopiridol呈浓度和时间依赖性抑制GIST882细胞增殖;Flavopiridol作用后GIST882细胞穿膜数量减少,侵袭能力降低,细胞凋亡率及G0/G1期比例增加,CDK1及CDK2蛋白的表达逐渐降低,caspase-3蛋白的表达逐渐增高。结论:Flavopiridol可抑制GIST882细胞增殖、降低侵袭力、阻滞细胞周期并诱导细胞凋亡,其抗肿瘤效应可能成为胃肠道间质瘤新的治疗途径。
Objective： To investigate the effect of Flavopiridol on the proliferation, invasion, apoptosis and cell cycle of gastrointestinal stromal tumors （ GIST ） cells GIST882.Method： GIST882 was cultured in vitro, flavopiridol was administered at different concentrations, MTT assay was used to detect cell proliferation, transwell chamber assay was used to detect cell invasion, flow cytometry was used to detect apoptosis and cell cycle, the expression of CDK1, CDK2 and caspase3 protein was detected by western-blot.Result： Flavopiridol inhibited the growth of GIST882 cells in a concentration-dependent and time-dependent way.Flavopiridol can reduce the ability of invasion, the apoptosis rate and GO/G1 phase ratio of GIST882 cells were increased after Flavopiridol treatment, the expression of CDK1 and CDK2 protein were decreased and caspase-3 protein was increased after Flavopiridol treatment.Conclusion： Flavopiridol can inhibit the proliferation of GIST882 cells, decrease the invasion capacities, block the cell cycle and induce apoptosis, the antitumor effect of Flavopiridol may be applicable on the treatment of gastrointestinal stromal tumors.
出处
《中国医学创新》
CAS
2017年第20期28-31,共4页
Medical Innovation of China
基金
山西省回国留学人员科研资助项目(2016-115)
