摘要
建立了利用双水杨醛邻苯二胺希夫碱(SALOPHEN)作为衍生剂结合高效液相色谱(HPLC)测定茶叶中Pb^(2+)含量的方法。Hypersil ODS2 C18反相色谱柱(250 mm×4.6 mm,5μm)为固定相,甲醇-水(80∶20,v/v)为流动相,检测波长为226 nm,反应体系pH为10.0。结果表明,样品中Pb^(2+)的线性范围为0.1~30 mg/L,线性相关系数为0.998 8,检出限为0.01 mg/L,加标回收率为91.87%~96.96%。该方法具有较高的灵敏度和稳定性,适用于茶叶中Pb^(2+)的检测。
The contents of Pb2+ in tea was determined by the high performance liquid chroma-tography (HPLC) method using bis (salicylaldehyde)-o-phenylenediamine (SALOPHEN) as derivatization reagent. The detection wavelength of ultraviolet was 226 nm with Hypersil ODS2 C-18 column (250 mmx4.6 mm, 5 mu m) as stationary phase, and pH 10.0 methanol. water (80 : 20, v/v) as mobile phase. The linear range of Pb2+ was 0.1-30 mg/L and the linear correlation coefficient was 0.998 8. The limit of detection was 0.01 mg/L with the recoveries of 91.87% -96.96%. The method has high sensitivity and stability with satisfactory results, which can be used for the detection of Pb2+ in tea samples.
出处
《色谱》
CAS
CSCD
北大核心
2017年第8期843-847,共5页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(21675138)~~
关键词
高效液相色谱
双水杨醛邻苯二胺希夫碱
重金属离子
茶叶
high performance liquid chromatography (HPLC)
bis(salicylaldehyde)-o-pheny-lenediamine
heavy metal ions
tea
