摘要
目的观察STZ诱导糖尿病大鼠胃平滑肌内质网应激相关蛋白GRP-78、CHOP、Caspase-12及其效应蛋白Bcl-2、Caspase-3的变化,探讨其在糖尿病胃轻瘫中发生的意义。方法根据STZ诱导6周大鼠出现糖尿病胃轻瘫症状及胃平滑肌细胞出现凋亡的前期实验结果,将SD大鼠分为正常对照组(NC)组和模型6周组(DM6W)。Western blot检测两组胃平滑肌GRP-78、CHOP、Caspase-12的表达,免疫组织化学染色检测两组胃平滑肌Bcl-2、Caspase-3的表达。结果 NC组胃平滑肌GRP-78、CHOP、Caspase-12的相对表达值低于DM6W组[(0.12±0.03)vs(0.23±0.03),(0.11±0.02)vs(0.24±0.03),(0.11±0.03)vs(0.24±0.04),P<0.01];NC组胃平滑肌Bcl-2的表达高于DM6W组[(491.91±22.96)vs(159.48±20.29),P<0.01];NC组胃平滑肌Caspase-3的表达低于DM6W组[(154.15±10.03)vs(489.45±52.08),P<0.01]。结论糖尿病胃轻瘫大鼠胃平滑肌存在内质网应激,并且参与诱导胃平滑细胞凋亡。
Objective To investigate the changes of GRP-78,CHOP,caspase-12,Bcl-2 and caspase-3 in gastric smooth muscle in STZ-induced diabetic rats,and explore their roles in the occurrence of diabetic gastroparesis. Methods According to the symptoms of diabetic gastroparesis and gastric smooth muscle apoptosis situation in STZ-induced 6W rats from our preliminary results,all the SD rats were divided into two groups：normal control group（NC） and model 6W group （DM6W）. The expressions of GRP-78,CHOP and caspase-12 in smooth muscle were tested by western blot method. The expressions of Bcl-2 and caspase-3 were measured by immuno-histochemical staining method. Results The expressions of GRP-78,CHOP and caspase-12 in smooth muscle were significantly lower in NC group than in DM6W group [（0.12±0.03） vs （0.23±0.03）,（0.11±0.02） vs （0.24±0.03）,（0.11±0.03） vs （0.24±0.04）,P〈0.01].Bcl-2 expression was significantly higher in NC group than in DM6W group [（491.91±22.96） vs （159.48±20.29）,P〈0.01]. Caspase-3 expression was significantly lower in NC group than in DM6W group [（154.15±10.03） vs （489.45±52.08）,P〈0.01]. Conclusion The endoplasmic reticulum stress existed in the gastric smooth muscle of rats with diabetic gastroparesis,which involved in the gastric smooth cell apoptosis.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2017年第7期626-629,共4页
Chinese Journal of Diabetes
基金
国家自然科学基金(81360070
81560142)
关键词
糖尿病
胃轻瘫
内质网
应激
意义
Diabetes mellitus
Gastroparesis
Endoplasmic reticulum
Stress
Significance
