摘要
目的探讨宁夏银川市居民SLC22A12基因rs1529909和rs7929627位点多态性与高尿酸血症的相关性。方法回顾性收集2014年4—11月在宁夏银川市某体检机构进行健康体检的365例确诊高尿酸血症患者资料,采用1∶1配对病例对照研究,按年龄(!5岁)、性别、民族进行匹配,选择同时间在该机构体检的365例血尿酸水平正常者作为对照。对研究对象进行问卷调查、体格检查、收集空腹静脉血进行生化检测和基因多态性检测。采用配对t检验进行两样本均数比较;条件Logistic回归分析高尿酸血症的影响因素;MDR软件分析两位点交互作用对高尿酸血症的影响。结果病例组体质指数、血尿酸、肌酐、总胆固醇、甘油三酯均值高于对照组(P<0.05)。rs1529909位点等位基因频率在男性病例组与对照组之间的差异有统计学意义(χ~2=4.887,P=0.027);rs7929627位点各基因型频率在回族病例组和对照组中的差异有统计学意义(χ~2=14.906,P=0.002),在男性病例组和对照组中的差异有统计学意义(χ~2=9.749,P=0.021)。交互作用分析结果显示,高危与低危基因型在两组之间的分布有统计学意义(χ~2=8.338,P=0.004),高危基因型发生高尿酸血症的风险是低危基因型的1.536倍(OR=1.536,95%CI 1.147~2.057)。结论rs1529909位点和rs7929627位点与男性高尿酸血症的发生均有关联;rs7929627位点与回族高尿酸血症的发生有关;rs1529909与rs7929627位点对于高尿酸血症的影响可能存在明显的拮抗作用。
Objective To investigate the relationship between SLC22A12 gene rs1529909 and rs7929627 polymorphism and hyperuricemia of residents in Yinchuan,Ningxia province. Methods Through retrospective analysis in healthy subjects from April to November in 2014,365 patients with hyperuricemia were selected. 1∶ 1 matched casecontrol study was carried out,matched with age（ ± 5 years old）,gender,race,and selected 365 people with normal serum uric acid levels as the control group. All subjects were investigated by questionnaire,had a physical examination,and collected fastingvenous blood for biochemical markers and gene polymorphism detection. Paired t test was used to compare the mean of two samples,and the influencing factors were analyzed by conditional logistic regression. The interaction of two sites on hyperuricemia was analyzed by MDR software. Results The body mass index,blood uric acid,creatinine,total cholesterol and triglyceride in the case group were higher than those in the control group,the difference was statistically significant（ P〈0. 05）. There was a significant differences between male cases and control group in the allele frequency of rs1529909（ χ^2= 4. 887,P = 0. 027）. There had a significant difference in the frequency of rs7929627 between the Hui case and control group（ χ^2= 14. 906,P = 0. 002）,also a significant difference between the male case and control group（ χ^2= 9. 749,P = 0. 021）. Additionally,theresult of interaction analysis showed that the distribution of high risk and low risk genotype had a statistically significant difference（ χ^2= 8. 338,P = 0. 004）. The risk of hyperuricemia with high genotype was 1. 536 times of those who had low-risk genotypes（ OR = 1. 536,95% CI1. 147-2. 057）. Conclusion rs1529909 and rs7929627 were associated with hyperuricemia among men. rs7929627 was related to it in the Hui. There may be a strong antagonistic effect on hyperuricemia between rs1529909 and rs7929627.
出处
《卫生研究》
CAS
CSCD
北大核心
2017年第4期589-594,共6页
Journal of Hygiene Research
基金
宁夏高等学校科学技术研究项目(No.NGY2014087)
关键词
高尿酸血症
基因多态性位点
hyperuricemia
single nucleotide polymorphism
