摘要
目的:探讨资木瓜总提物中莽草酸(Shikimic acid,SA)对C48/80(Compound 48/80)刺激大鼠腹腔肥大细胞(Rat peritoneal mast cells,RPMC)脱颗粒的影响及抗炎作用。方法:HPLC法进行资木瓜总提物中莽草酸成分的定性分析;采用甲苯胺蓝(Toluidine blue,TB)、免疫组化及电镜法进行RPMC鉴定;CCK-8法检测各浓度组RPMC细胞的增殖情况;底物法检测β-己糖苷酶的释放率;ELISA法检测细胞上清中组胺的含量。结果:SA在0~90μg/ml时,对RPMC生长无显著影响;与阳性对照组比较,SA能有效抑制β-己糖苷酶的释放和抑制组胺的分泌。结论:SA通过抑制C48/80刺激的RPMC脱颗粒而抑制炎症介质组胺的释放,进而发挥抗炎作用。
Objective： To investigate the effect of Chaenomeles speciosa total extract shikimic acid （SA） on the degr.anulation of compound 48/80 （C48/80） stimulated rat peritoneal mast cells and the anti-inflammatory. Methods： Qualitative analysis of shikimic acid in the total extract of Chaenomeles speciosa by high performance liquid phase; RPMC were identified by toluidine blue, immunohis- tochemistry and electron microscopy; CCK-8 method was used to detect the proliferation of RPMC in each concentration group. The release rate of β-hexosidase was determined by substrate method. The content of histamine in supernatant was detected by ELISA. Results： SA had no significant effect on the growth of RPMC at the concentration of 0-90 μg/ml. Compared with the positive control group, SA could effectively inhibit the release of β-hexosidase and inhibit the secretion of histamine. Conclusion： SA inhibits the release of histamine in inflammatory mediators by inhibiting the degranulation of RPMC stimulated by CA8/80, and then exerts anti- inflammatory effects.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第7期1035-1038,共4页
Chinese Journal of Immunology
基金
三峡大学医学院国家自然科学基金资助项目(No.81673665
81274116)
关键词
资木瓜
莽草酸
RPMC
β-己糖苷酶
组胺
Chaenomeles speciosa
Shikimic acid
RPMC
β-hexosl minidase
Histamine
