摘要
目的:研究ECRG4基因在胃腺癌组织、癌旁组织及正常胃组织中的表达差异及其与患者临床病理特征的相关性。方法:收集手术切除并经病理证实的胃腺癌组织石蜡标本58例作为胃癌组,癌旁组织及正常胃组织各15例作为癌旁组、正常组,用免疫组织化学SP法检测胃腺癌组织与癌旁及正常组织中ECRG4编码蛋白的表达情况,用SPSS19.0统计软件对ECRG4蛋白的表达差异及其与患者的临床病理特征进行统计学分析。结果:ECRG4蛋白在胃癌组中阴性表达29例、弱阳性表达29例、未见阳性及强阳性表达;癌旁组织中阴性表达0例、弱阳性表达5例、阳性表达7例、强阳性表达3例;正常胃组织中阴性表达1例、弱阳性表达4例、阳性表达2例、强阳性表达8例。三者E-CRG4蛋白表达差异具有统计学意义(P<0.001)。两两比较后认为正常胃组织与胃癌组织、胃癌组织与癌旁组织E-CRG4蛋白表达差异具有统计学意义(P<0.05),而正常胃组织与胃癌癌旁组织ECRG4蛋白表达差异不具有统计学意义(P>0.05)。ECRG4的表达与胃癌患者的分化程度有关,分化程度越低者其表达缺失率越高(P<0.05);ECRG4蛋白表达与病人年龄、性别、肿瘤发生部位、TNM分期及是否淋巴结转移无关(P>0.05)。结论:ECRG4蛋白在胃腺癌组织中表达明显下调或缺失,而在胃正常组织中普遍表达。ECRG4蛋白表达与患者肿瘤的分化程度有关,分化程度越低者其表达缺失率越高。
Objective To study the gene ECRG4 in gastric adenocarcinoma and its expression differences with the patient of the clinical and pathological features of relevance.Methods Collect surgical resection and confirmed by pathology gastric adenocarcinoma tissue of paraffin wax 58 cases of cancer,15 cases of the normal tissue and 15 cases of the adjacent normal gastric cancer tissue,using immunohistochemical method to detect the ECRG4 coding protein expression,with SPSS19.0 statistical software to ECRG4 protein expression differences and their patients and the clinical and pathological features of the statistical analysis.Results ECRG4 protein in the stomach cancer in 29 cases of negative expression,and the weak positive express 29 patients,has not seen the positive and strong positive expression;Cancer adjacent tissues negative express 0 example,weak positive in 5 cases,and the masculine expression expression in 7 cases,strong positive expression in 3 patients,Normal in stomach tissue negative expression in 1,4 cases were weak positive expression,and the masculine expression in 2 cases,strong positive 8 cases of expression.The three ECRG4 protein expression differences are statistically significant.After comparing two two think is normal stomach tissue and gastric cancer tissue,stomach tissue and cancer tissue adjacent to ECRG4 protein expression are statistically significant differences,but the normal stomach tissue and cancer of the stomach cancer tissue adjacent to ECRG4 protein expression differences were not significant.ECRG4 expression and gastric cancer patients about the differentiation degree,the low differentiated the lack of the higher rate of expression;ECRG4 protein expression and patient age,sex,tumor location,TNM staging and whether lymph node metastasis have nothing to do.Conclusion ECRG4 protein expression in the tissue in gastric adenocarcinoma is obviously down-regulated or missing,and in normal tissue in the stomach common expression.ECRG4 protein expression and cancer differentiation degree of relevant,differentiation degree the low loss rate is high its expression.
出处
《湖南师范大学学报（医学版）》
2012年第1期69-73,共5页
Journal of Hunan Normal University(Medical Sciences)
关键词
ECRG4
抑癌基因
胃腺癌
免疫组织化学
ECRG4
tumor-suppressor genes
gastric adenocarcinoma,immunohistochemical
