摘要
目的:探讨云南藤黄提取物抑制裸鼠大肠癌植性实体瘤的作用机制。方法:建立裸鼠人结肠癌皮下移植瘤模型,随机分为:模型对照组、阳性对照组(5-氟脲嘧啶)、云南藤黄提取物低、中、高剂量组。连续治疗28 d,于末次给药后12 h,剥取肿瘤,称重,计算抑瘤率;并检测肿瘤细胞凋亡情况;免疫组化法检测肿瘤组织中cyclin E1,c-Met和hnRNPK的表达。结果:云南藤黄提取物低、中、高剂量对肿瘤的抑瘤率分别为12.5%、20.83%和29.17%,与空白对照组相比,差异有统计学意义(P<0.01)。肿瘤细胞凋亡率增加,并且随着云南藤黄提取物浓度的增加而增加,具有剂量依赖性(P<0.05)。云南藤黄提取物能明显降低肿瘤组织中cyclin E1,c-Met和hnRNPK的表达,具有剂量依赖性(P<0.01)。结论:云南藤黄具有较好的体内抗肿瘤作用,可能是通过降低cyclin E1,c-Met和hnRNPK的表达,抑制肿瘤细胞在体内增殖。
Objective：To observe the mechanism of garcinia yunnanensis extract on subcutaneously implanted tumor of human colorectal carcinoma in nude mouse.Methods：A nude mouse model of subcutaneously implanted tumor of human colorectal carcinoma was established,and experimental nude mice were randomly divided into 5 groups：model group,positive control group （5-Fluorouracil）,and high/middle/low doses of garcinia yunnanensis group.After 28 days of treatment,the tumor was cut to weigh and anti-tumor rate was calculated,and the apoptosis ratio of tumor cells was analyzed.CyclinE1,c-Met,hnRNPK expression level were analyzed by immuno-histochemical method.Results：The experimental results showed that,the anti-tumor rates in low/middle/high doses of garcinia yunnanensis group were 12.5%,20.83% and 29.17%,which were significantly different from the normal control group （P〈0.01）.The apoptosis rate of tumor cells was increased with the increasing of extract concentration of garcinia yunnanensis,which showed a dose-dependent manner （P〈0.05）.Garcinia yunnanensis could significantly reduce the expression of cyclinE1,c-Met and hnRNPK with a dose-dependent manner （P〈0.01）.Conclusion：Garcinia yunnanensis has an obvious anti-tumor effect in vivo,and it may inhibit the proliferation of tumor cells in vivo by reducing the expression of cyclinE1,c-Met and hnRNPK.
出处
《世界中医药》
CAS
2017年第2期377-381,共5页
World Chinese Medicine
基金
国家自然科学基金面上项目(编号:81173485)--藤黄属植物抗肿瘤活性成分及其作用机理研究
国家自然科学基金青年项目(编号:81603344)--怒江藤黄中Nujiangexanthone A抗肿瘤作用机制研究
新疆自治区科技支疆项目(编号:2013911125)
