摘要
目的探讨氯化镧(LaCl_3)暴露对仔鼠海马紧密连接蛋白表达和基因转录水平影响。方法将40只Wistar孕鼠随机分为对照组(蒸馏水)和0.25%、0.50%、1.00%(w/v)氯化镧组,从仔鼠出生起孕鼠通过饮水染毒LaCl_3,断乳后仔鼠继续饮用原浓度LaCl_3溶液1个月;采用Western blot法检测仔鼠海马claudin-5、occludin和紧密连接蛋白-1(ZO-1)蛋白表达,real-time PCR法检测仔鼠海马Cldn5、Ocln和Tjp1 mRNA表达。结果与对照组比较,0.50%和1.00%氯化镧组仔鼠海马claudin-5蛋白表达水平[分别为(0.059±0.007)、(0.041±0.003)]与occludin蛋白表达水平[分别为(0.085±0.004)、(0.067±0.004)]均明显降低(P<0.05);1.00%氯化镧组仔鼠海马Cldn5、Ocln和Tjp1 mRNA表达水平分别为(0.743±0.026)、(0.900±0.119)、(0.931±0.100),与对照组比较,1.00%氯化镧组仔鼠海马Cldn5 mRNA表达下降(P<0.05)。结论生长发育期暴露氯化镧可下调仔鼠海马claudin-5和occludin表达,导致血脑屏障紧密连接受损。
Objective To observe the effect of lanthanum chloride （LaCl3） exoposure on tight junction proteins expression and genes transcription in hippocampus of offspring rats.Methods Forty pregnant Wistar rats were randomly divided into a control group and three LaCl3 treated groups with 0.25%,0.5%,1.0% （w/v） of LaCl3 in drinking water.The offspring rats were exposed to LaCl3 through maternal milk during lactation,and through drinking water for one month after delectation.Claudin-5 （Cldn5）,occludin （Ocln）,and zonula occludens-1 （ZO-1） protein expression in hippocampus were measured with Western blot;while Cldn5,Ocln,and tight junction protein 1 （Tjp1） mRNA level in hippocampus were detected with real-time PCR.Results There were significant reductions in protein expression of claudin-5 （0.059±0.007 vs.0.085±0.004） and Ocln （0.041±0.003 vs.0.067±0.004） in hippocampus tissues of the offspring rats exposed to 0.5% and 1.0% LaCl3 compared to those of the controls （both P〈0.05）.In addition,Cldn5,Ocln and Tjp1 mRNA level in in hippocampus tissues of the offspring rats exposed to 1.0% LaCl3 were 0.743±0.026,0.900±0.119,and 0.931±0.100,respectively,and there was only a significant difference in Cldn5 mRNA level compared to the control group.Conclusion LaCl3 exposure during development period down-regulates the expression of claudin-5 and occludin,thus causes damage of blood-brain barrier tight junctions in hippocampus of offspring rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2017年第7期1079-1082,共4页
Chinese Journal of Public Health
基金
国家自然科学基金(81502837)
辽宁省科技厅基金(2015020367)
关键词
氯化镧
海马
血脑屏障
紧密连接
lanthanum chloride
hippocampus
blood-brain barrier
tight junction
