摘要
CBL基因在植物逆境应答过程中具有重要作用,但在西瓜作物的抗逆育种研究中鲜有相关报道。该研究利用生物信息学的分析方法从已发表的西瓜全基因组中鉴定出7个CBL基因(ClaCBL1~ClaCBL7),并分析了它们在全基因组范围内的分布情况、分子结构特征、顺式元件以及系统进化等基本特征,为进一步研究西瓜CBL基因的功能提供参考。结果表明:西瓜CBL基因在全基因组中的分布是不均匀的,其中仅ClaCBL4基因有9个外显子,其余基因均为8个外显子,编码区序列大小在639~738bp。在进化上西瓜CBL基因分为3个不同的类群;它们编码区域内的氨基酸序列均含有能与CIPK互作的FSPF高保守性位点及能与钙离子结合的3个EF-hand结构;除了ClaCBL1预测定位在胞外基质中,其余的ClaCBL定位在细胞内的不同部位;在西瓜CBL基因启动子上游的序列中存在多个应答不同逆境和植物激素的顺式元件,而且CBL家族不同成员基因含有的顺式元件的种类和数目各不相同。上述分析显示,西瓜CBL可能参与多种生物学过程,而且不同成员存在功能上的分化。
Plant CBLfamily genes play important roles in response to environmental stimuli.However,the available knowledge of CBL in watermelon is insufficient.In this study,seven CBL genes（ClaCBL1-ClaCBL7） were annotated in watermelon genome by bioinformatics method and their genomic distribution,molecular feature,phylogeny and cis-elements were also analyzed for providing valuable information for further functional dissection of watermelon CBLgenes.The results showed that watermelon CBLgenes were unevenly distributed in genome,and only one（ClaCBL4）had nine exons and the rest of them had eight exons.The putative coding sequence length of CBLranges from 639 bp to 738 bp.On the basis of phylogeny,they were divided into three groups.Their coded proteins had FPSF motif,which interacted with CIPK kinases,and three EF-hand motifs,which could bind calcium ions.The putative proteins of ClaCBL were all localized into different subcellular locations except ClaCBL1 in extracellular matrix.In addition,there are several cis-elements existed in upstream sequences of watermelon CBL genes,which could response to different environmental stimuli and phytohormones.And the cis-element types and number of different watermelon CBLgenes were not identical.These results indicated that watermelon CBL genes might involve in multiple biological processes and they had different functions.
出处
《北方园艺》
CAS
北大核心
2017年第15期18-24,共7页
Northern Horticulture
基金
国家自然科学基金面上资助项目(31572144)
国家西甜瓜产业技术体系-分子育种岗位资助项目(CARS-26-02)
