摘要
采用硅胶柱色谱、sephadex LH-20凝胶柱色谱、制备HPLC等多种色谱技术,从狭叶锦鸡儿(Caragana stenophylla)根80%乙醇提取物的二氯甲烷部位中分离得到14个单体化合物,依据理化性质及波谱数据分别鉴定为:2-(4-hydroxy-3-methoxylphenyl)-3-methoxylbenzofuran-6-ol(1)、mucodianin C(2)、羟基二甲氧苯基苯并呋喃醇(isopterofuran,3)、芒柄花素(formononetin,4)、阿夫罗摩辛(afromosin,5)、毛蕊异黄酮(calycosin,6)、刺槐素(acacetin,7)、3-O-甲基山柰酚(3-O-methylkaempferol,8)、甘草素(liquiritigenin,9)、异甘草素(isoliquiritigenin,10)、variabilin(11)、白藜芦醇(resveratrol,12)、浙贝素(zhebeiresinol,13)、2,3-dicarboxy-6,7-dihydroxy-1-(3',4'-dihydroxy)-phenyl-1,2-dihydronaphthalen(14)。其中化合物1是新化合物(苯并呋喃衍生物类化合物),命名为mucodianin S,化合物2,3,11,13,14为首次从锦鸡儿属中分离得到,化合物4~10为首次从狭叶锦鸡儿中分离得到。采用MTT比色法对部分单体化合物进行体外抗肿瘤活性测试,实验结果表明化合物2对人类肿瘤细胞系Hep G2,He La具有显著的生长抑制活性,其IC50分别为(16.18±0.95),(3.75±0.08)μmol·L-1。
Fourteen compounds were isolated from the 80% ethanol extract of Caragana stenophylla root, by using a combination of various chromatographic approaches, including silica gel sephadex LH-20 column chromatography, and preparative HPLC. On the ba- sis of their physical and chemical properties and spectroscopic data, their structures were elucidated as 2-（4-hydroxy-3-methoxy lphe- nyl）-3-methoxyl benzofuran-6-ol （1） , mucodianin C （2） , isopterofurau （3） , formononetin （4） , afromosin （5） , calycosin （6） , acacetin （7）, 3-O-methylkaempferol （8）, liquiritigenin （9）, isoliquiritigenin （ 10）, variabilin （ 11 ）, resveratrol （ 12）, zhebeires- inol （13）, and 2, 3-dicarboxy-6, 7-dihydroxy-1-（3＇, 4＇-dihydroxy）-phenyl-1, 2-dihydronaphthalen （14）. Compound 1 is a new benzofuran derivative, named as mucodianin S ; compounds 2, 3, 11, 13, 14 were isolated from the genus Caragana for the first time, and compounds 4-10 were firstly isolated from Caragana stenophylla. MTF assay was used to determine their cytotoxicity of the isolatedcompounds against human tumor cell lines, and 2 showed cytotoxicity against human hepato cellular cancer （HepG2） and human cervi- cal （HeLa） lines, with IC50 values of （16. 18 ±0. 95 ） , （3.75 ± 0. 08 ）μmol . L-1, respectively.
作者
苏比努尔.买乌兰
潘兰
贾新岳
张涛
贾晓光
邹忠梅
SUBINUER Maiwulan PAN Lan JIA Xin-yue ZHANG Tao JIA Xiao-guang ZOU Zhong-mei(School of Life Science and Technology, Xinjiang University, Urumqi 830046, China Xinjiang Institute of Chinese Medicine and Ethnic Drug, Urumqi 830002, China Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第14期2697-2703,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金新疆联合基金项目(U1203202)
关键词
狭叶锦鸡儿
化学成分
结构鉴定
抗肿瘤活性
Caragana stenophylla
chemical constituents
structure identification
anti-tumor activity
