摘要
目的探究羊膜泪道支架对雄兔干眼症的预防效果。方法选取36只健康雄兔,剪除第三眼睑并抗感染处理,随机分为3组(每组12只),制作去势雄兔模型,其中A组为阴性对照组,B组为干眼模型组,C组为泪道羊膜预防组,并在植入羊膜泪道支架前、植入后2周、4周及6周行角膜荧光素(fluorescent,FL)染色、Schirmer I试验、Western blot、免疫荧光染色及角膜共聚焦显微镜检查。结果三组不同时间点间的泪液分泌量及FL有明显改变(F=7.126,P=0.009;F=9.658,P=0.016),三组间泪液分泌量及FL有明显改变(F=12.582,P=0.005;F=13.187,P=0.013),三组的泪液分泌量及FL变化趋势也有明显改变(F=8.531,P=0.007;F=10.652,P=0.019)。A、B、C三组植入后6周上皮基底细胞分别(3811±414)个细胞、(3820±314)个细胞、(2789±353)个细胞,炎症细胞密度分别为(266±28)个细胞、(266±29)个细胞、(67±13)个细胞;A、B、C三组植入后6周上皮基底细胞(F=13.442,P=0.012)和炎症细胞密度(F=9.231,P=0.021)比较,差异均有统计学意义。泪道上皮K16染色均为阴性,植入羊膜泪道支架后各时间点A、B、C三组兔泪小管组织中α-SMA表达无明显改变,差异有统计学意义(F=14.681,P=0.002)。结论植入羊膜泪道支架对雄兔干眼症的预防有一定的效果。
Objective To explore the effects of amniotic lacrimal duct stenting on the prevention of dry eye in castrated rabbits.Methods Thirty-six healthy male rabbits were selected,the third eyelid were cut off and anti-infection treatment were given,which were randomly divided into 3 groups （12 cases in each group）,the castrated male rabbits models were made.Among them,group A was negative control group,group B was dry eye model group,group C was group of lacrimal amniotic membrane group.At 2 weeks before implantation of amniotic lacrimal duct stent,2 weeks,4 weeks and 6 weeks after implantation,the fluorescent（FL）examination,Western blot,Schirmer I examination,immunofluorescence staining and corneal confocal microscopy were performed.Results The levels of tear secretion and FL in the three groups among different time points were significantly different （F=7.126,P= 0.009;F=9.658,P=0.016）,and there were significant differences among three groups （F=12.582,P=0.005;F=13.187,P=0.013）.The tendency of tear secretion and FL in the three groups were also significantly changed （F=8.531,P=0.007;F=10.652,P=0.019）.The epithelial basal cells at 6 weeks after implantation in three groups were 3811±414,3820±314,2789±353,and the density of inflammatory cells was 266 ± 28,266 ± 29,67±13,there were significant differences among three groups （F=13.442,P=0.012;F=9.231,P=0.021）.The K16 staining in the duct epithelium were negative,and the expression of α-SMA in the lacrimal duct tissue of group A,B and C was not changed at all time points after implantation of amniotic lacrimal stent,and there was no significant difference （F=14.681,P=0.002）.Conclusion The amniotic lacrimal stent implantation has certain effect on the prevention of dry eye in rabbit.
出处
《眼科新进展》
CAS
北大核心
2017年第8期709-713,共5页
Recent Advances in Ophthalmology
基金
国家自然科学基金资助(编号:81660158
81160118
81160148
81460092
81400372
81400424)
江西省远航工程(编号:2014022)
江西省自然科学基金重大项目(编号:2016ACB21017)
江西省青年科学基金(编号:20151BAB215016
20161BAB215198)
江西省重点研发项目(编号:20151BBG70223)
江西省教育厅重点项目(编号:GJJ160020)
江西省教育厅科技计划项目(编号:GJJ160122)
江西省卫计委科技计划项目(编号:20164017
20175115
20175116)
江西省卫计委中医药科技计划项目(编号:20150823)~~
关键词
羊膜泪道支架
干眼症
去势雄兔
amniotic lacrimal duct stent
xerophthalmia
castrated rabbits
